1.13.11.11: tryptophan 2,3-dioxygenase

This is an abbreviated version!
For detailed information about tryptophan 2,3-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.11

Reaction

L-tryptophan
+
O2
=
N-formyl-L-kynurenine

Synonyms

33737, BRAFLDRAFT_210874, C28H8.11, hTDO, IDO-1, IDO-2, TDO, TDO2, TDOa, v1g157887, vCG5163, XcTDO

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.11 tryptophan 2,3-dioxygenase

Engineering

Engineering on EC 1.13.11.11 - tryptophan 2,3-dioxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F140A
-
site-directed mutagenesis, the mutant shows 0.25% of wild-type activity
F72A
-
site-directed mutagenesis, inactive mutant
H328A
-
site-directed mutagenesis, inactive mutant
H76A,
-
site-directed mutagenesis, inactive mutant
R144A
-
site-directed mutagenesis, the mutant shows 0.88% of wild-type activity
S151A
-
site-directed mutagenesis, the mutant shows 9.08% of wild-type activity
Y175G
-
site-directed mutagenesis, the mutation leads to a 6fold slower multiple turnover velocity. In addition, pre-incubation of the Y175G mutant with 8 mM NFK retards the formation of the ternary complex by about 100fold and impedes Trp binding
Y42A
-
site-directed mutagenesis, the mutant shows 0.5% of wild-type activity
Y45A
-
site-directed mutagenesis, the mutant shows 1.13% of wild-type activity
additional information