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1.13.11.1: catechol 1,2-dioxygenase

This is an abbreviated version!
For detailed information about catechol 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.1

Reaction

catechol
+
O2
=
cis,cis-muconate

Synonyms

(chloro-)catechol 1,2-dioxygenase, 1,2 CTD, 1,2-CTD, 1,2-pyrocatechase, 3-chlorocatechol 1,2-dioxygenase, BC-C23O, C1,2-DO, C1,2O, C12, C12D, C12DO, C12O, cat12, CatA, catechase, catechol 1,2 dioxygenase, catechol 1,2-dioxygenase, catechol 1,2-dioxygenase type I, catechol 1,2-dioxygenase type II, catechol 1,2-oxygenase, catechol dioxygenase, catechol oxygenase, catechol-oxygen 1,2-oxidoreductase, catechol:oxygen 1,2-oxidoreductase, CD I, CD II, CD-I, CD-II, CD-III-1, CD-III-2, CDI1, CDI2, chlorocatechol 1,2-dioxygenase, clCA, extradiol dioxygenase MhpB, IsoB, putative catechol 2,3-dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, TfdC

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.1 catechol 1,2-dioxygenase

Purification

Purification on EC 1.13.11.1 - catechol 1,2-dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
about 18fold after ammonium sulfate precipitation, DEAE-celullose, Sephadex G-150 and hydroxylapatite chromatographie
-
ammonium sulfate precipitation and Hitrap QFF column chromatography
-
CD I and CD II respectively
-
cells are disrupted and centrifugates, protein supernatant is separated by 2D gel electrophoresis
-
DEAE-Toyopearl column chromatography, phenyl Sepharose CL-4B column chromatography, and Resource Q column chromatography
-
four different isoenzymes: CD-I, DC-II, CD-III-1, CD-III-2
-
from aniline-grown cells and from L-malate-grown cells
-
isoenzymes A and B
-
native enzyme partially by anion exchange chromatography
-
native enzyme to homogeneity from cell supernatant by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, folloed by gel filtration
-
native isozymes 1-3 by ammonium sulfate fractionation, and affinity and anion exchange chromatography
-
Ni-NTA resin column chromatography
-
partial purification with HiTrap Q Sepharose column chromatography
-
partially, two active fractions
-
pyrocatechase I and II respectively
-
Q-Sepharose column chromatography, gel filtration
Q-Sepharose column chromatography, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration