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1,10-phenanthroline
-
progressive inactivation, loss of all activation after 90 min, reactivation with Fe3+
2,2'-dipyridyl
complete inhibition at 2 mM, 99.9% at 1 mM
2,4-Dichlorophenol
26.0% inhibition at 0.1 mM, 29.8% at 0.3 mM
2-chlorophenol
31.1% inhibition at 0.1 mM, 17% at 0.3 mM
2-methylphenol
24.7% inhibition at 0.1 mM
3,4,5,6-tetrachlorocatechol
competitive inhibitor
3,4,5-trichlorocatechol
-
competitive inhibitor
3,5-dichlorocatechol
competitive inhibitor
3-methylphenol
20.4% inhibition at 0.1 mM, 32% at 0.3 mM
3-methylthiocatechol
-
competitive inhibitor
4-Chlorophenol
11% inhibition at 0.1 mM, 13% at 0.3 mM
4-Methylphenol
8% inhibition at 0.1 mM, 27.5% at 0.3 mM
alpha,alpha'-dipyridyl
-
94% inhibition, 1 mM
alpha-chloro-3,4-dihydroxyacetophenone
-
-
catechol
substrate inhibition at over 0.08 mM substrate concentration
Co2+
Stutzerimonas stutzeri
1.3 mM, 30 min, 24% loss of activity
CuCl2
-
0.1 mM, 41% inhibition
CuSO4
-
0.1 mM, 10 min, complete inhibition
FeCl3
-
0.1 mM, 14% inhibition
FeSO4
-
0.1 mM, 10 min, 12% inhibition
H2O2
-
0.1 mM, 10 min, 98% inhibition
mercaptoethanol
-
0.1 mM, 10 min, 98% inhibition
NaCl
Stutzerimonas stutzeri
increasing NaCl concentration gradually reduce activity. In 0.7 M NaCl quaternary structur changes to a dimeric arrangement and causes a 51% decrease in specific activity on catechol substrate
p-hydroxymercuribenzoate
-
-
PbSO4
-
0.1 mM, 75% loss of activity
Phenanthroline
complete inhibition at 3 mM, 99% at 1 mM
SDS
5 mM, complete loss of activity
Sodium dithionite
-
1 mM, 36% loss of activity
streptomycin sulfate
-
5%, 10 min, 80% inhibition
tetrachlorocatechol
-
markedly inhibitory at concentration above 4 microM
Triton X100
5 mM, about 90% loss of activity
Tween 20
5 mM, about 50% loss of activity
additional information
-
not inhibited by Hg2+
-
2-mercaptoethanol
-
-
3-methylcatechol
-
-
3-methylcatechol
-
at concentration at 20 microM and above
4,5-dichlorocatechol
-
competitive inhibitor
4,5-dichlorocatechol
competitive inhibitor
4-methylcatechol
-
-
4-nitrocatechol
-
-
Ag+
-
-
Ag+
-
0.5 mM, complete inhibition
Ag+
-
inhibition can almost completely be reversed by addition of glutathione
AgNO3
-
0.001 mM, 93% loss of activity
AgNO3
-
0.1 mM, 10 min, complete inhibition
AgNO3
-
0.1 mM, enzyme from aniline-grown cells loses 88% of its activity, enzyme from L-malate-grown cells loses 67% of its activity
Al3+
-
0.01 M, 100%
Al3+
-
inhibits 16.5% at 3 mM
Ca2+
-
58% residual activity at 0.5 mM
Ca2+
5 mM, about 25% loss of activity
Ca2+
Stutzerimonas stutzeri
1.3 mM, 30 min, 39% loss of activity
Cu2+
-
-
Cu2+
-
1 mM, inhibits the immobilized enzyme by 60%, the free enzyme by 40%
Cu2+
-
0.5 mM, 81% inhibition
Cu2+
-
pyrocatechase I: in absence of 2-mercaptoethanol, pyrocatechase II: with 2-mercaptoethanol, 20 hours incubation time
Cu2+
Stutzerimonas stutzeri
1.3 mM, 30 min, 42% loss of activity
EDTA
-
50% residual activity at 0.5 mM
EDTA
-
1 mM, 10 min, 20% inhibition
EDTA
99.1 inhibition at 3 mM, 93.7% at 1 mM
EDTA
Stutzerimonas stutzeri
1.3 mM, 30 min, 29% loss of activity
Fe2+
-
weak inactivation
Fe2+
Stutzerimonas stutzeri
1.3 mM, 30 min, 20% loss of activity
Fe3+
-
-
Fe3+
-
inhibits 42% at 3 mM
Fe3+
Stutzerimonas stutzeri
1.3 mM, 30 min, 96% loss of activity
Hg2+
-
-
Hg2+
-
inhibition can almost completely reserved by addition of glutathione
Hg2+
Stutzerimonas stutzeri
1.3 mM, 30 min, complete loss of activity
HgCl2
-
0.1 mM, 64% inhibition
HgCl2
-
1 mM, 52% inhibition
HgCl2
-
enzyme from aniline-grown cells completely loses activity, enzyme from L-malate-grown cells loses 98% of its activity
iodoacetic acid
-
CD I only
K+
-
1 mM, inhibits the free enzyme by 38%, no inhibition of the immobilized enzyme by K+
K+
Stutzerimonas stutzeri
1.3 mM, 30 min, 28% loss of activity
KCN
-
53% inhibition, 1 mM
Mg2+
5 mM, about 20% loss of activity
Mg2+
-
1 mM, inhibits the free enzyme by 9%, no inhibition of the immobilized enzyme by Mg2+
Mg2+
Stutzerimonas stutzeri
1.3 mM, 30 min, 40% loss of activity
Mn2+
5 mM, about 60% loss of activity
Mn2+
-
0.5 mM, 64% inhibition
Mn2+
-
inhibits 27% at 3 mM
Mn2+
Stutzerimonas stutzeri
1.3 mM, 30 min, 51% loss of activity
NH4+
-
10 mM, inhibits the immobilized enzyme by 40%, the free enzyme by 50%
Ni2+
-
0.01 M, 85%
Ni2+
-
complete inhibition at 3 mM
Ni2+
Stutzerimonas stutzeri
1.3 mM, 30 min, 28% loss of activity
o-phenanthroline
-
-
o-phenanthroline
-
CD I and CD II respectively
o-phenanthroline
-
0.05 mM, 10 min, 3% inhibition
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
CD I only
p-chloromercuribenzoate
-
inhibition can almost completely be reversed by addition of glutathione
p-chloromercuribenzoate
-
64% inactivation, 5 mM, 30 min, 24°C
p-chloromercuribenzoate
-
pyrocatechase I: in absence of 2-mercaptoethanol, pyrocatechase II: with 2-mercaptoethanol, 20 hours incubation time
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
PCMB
-
0.5 mM, complete inhibition
PCMB
-
0.1 mM, 16% inhibition
PCMB
-
1.0 mM, enzyme from aniline-grown cells loses 20% of its activity, enzyme from L-malate-grown cells loses 10% of its activity
Tiron
-
-
Tiron
-
56% inhibition, 1 mM
Tiron
-
48% inactivation, 5 mM, 30 min, 24°C
Tiron
-
0.5 mM, 20% loss of activity
Zn2+
5 mM, complete loss of activity
Zn2+
Stutzerimonas stutzeri
1.3 mM, 30 min, 92% loss of activity