1.12.99.6: hydrogenase (acceptor)

This is an abbreviated version!
For detailed information about hydrogenase (acceptor), go to the full flat file.

Word Map on EC 1.12.99.6

Reaction

H2
+
acceptor
=
reduced acceptor

Synonyms

ECH, Ech hydrogenase, EchA, EchB, EchD, EchE, EchF, endo-hydrogenase, exo-hydrogenase, F420-reducing [NiFe] hydrogenase, factor420 hydrogenase, Fe-hydrogenase, Fe-only hydrogenase, ferredoxin hydrogenase, H2 producing hydrogenase [ambiguous], H2-sensing [NiFe] hydrogenase, Hfs, Hmd, HoxEFUYH type [NiFe] hydrogenase, HoxG, HupL, HupS, hupSLW, Hya, HyaA, HyaB, Hyb, HybA, HycE, Hyd X, Hyd-1, Hyd-2, HYD1, hydA, HydA1, HydA2, HydB, hydrogen dehydrogenase, hydrogen uptake hydrogenase, hydrogen-forming methylenetetrahydromethanopterin dehydrogenase, hydrogen-lyase, hydrogen-lyase [ambiguous], hydrogen:ferredoxin oxidoreductase, hydrogen:methylviologen oxidoreductase, hydrogenase (ferredoxin), hydrogenase 1, hydrogenase 2, hydrogenase 3, hydrogenase I (bidirectional), hydrogenase II, hydrogenase II (uptake), hydrogenase-1, hydrogenase-Fe-S, hydrogenlyase [ambiguous], HyhL, HynSL, HynSL hydrogenase, Hyq, iron-sulfur-cluster-free hydrogenase, Mbh, membrane-bound [NiFe]-hydrogenase, methyl viologen-reducing hydrogenase, methylviologen hydrogenase, Ni-Fe hydrogenase, nickel-iron hydrogenase, NiFe hydrogenase, NiFe-hydrogenase, NIFeSe-hydrogenase, regulatory hydrogenase, respiratory hydrogenase, SHI, uptake hydrogenase, uptake hydrogenase [ambiguous], uptake [NiFe] hydrogenase:, uptake [NiFe]-hydrogenase, [FeFe] hydrogenase, [FeFe]-H2ase, [FeFe]-hydrogenase, [FeFe]H2ase, [Fe] hydrogenase, [Fe]-hydA, [Fe]-hydrogenase, [Ni-Fe] hydrogenase, [NiFeSe]-hydrogenase, [NiFe] hydrogenase, [NiFe]-hydrogenase, [NiFe]-hydrogenase 2, [NiFe]-hydrogenase-2, [NiFe]H2ase, [NiFe]hydrogenase

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.99 With unknown physiological acceptors
                1.12.99.6 hydrogenase (acceptor)

Specific Activity

Specific Activity on EC 1.12.99.6 - hydrogenase (acceptor)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.008
-
hydrogen uptake specific activity of the hydrogenase 3 lacking mutant enzyme HD705/pBS(Kan), using methyl viologen as a substrate
0.035
-
hydrogen uptake specific activity of the wild type enzyme, using methyl viologen as a substrate
0.107
-
crude extract
0.4 - 0.7
-
H2 evolution
1.92
-
methyl viologen reduction by H2
2
-
crude extract, at 55C, pH 7.0, using methylene blue as electron acceptor
14.6
-
purified enzyme, proton reduction activity
14.8
-
2.4fold purified enzyme, hydrogen oxidation activity
30
-
after 152fold purification, at 55C, pH 7.0, using dichloroindophenol as electron acceptor
55
-
after 152fold purification, at 55C, pH 7.0, using methyl viologen as electron acceptor
80
-
Cr6+ reduction; H2 uptake
90
-
H2 formation with reduced ferredoxin
110
-
purified enzyme
114
-
acceptor methyl viologen
150
-
at pH 6.0 and 25C
161
-
Cr6+ reduction
230
-
H2 evolution
300
-
; acceptor benzyl viologen
332
-
H2 uptake
344
-
after 152fold purification, at 55C, pH 7.0, using methylene blue as electron acceptor
484
-
after 152fold purification, at 80C, pH 7.0, using methylene blue as electron acceptor
624
-
acceptor 2,3-dimethylnaphthoquinol
1078
-
after 152fold purification, at 55C, pH 7.0, using phenazine methosulfate as electron acceptor
7680
-
Cr6+ reduction
50000
-
H2 uptake
additional information