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1.12.99.6: hydrogenase (acceptor)

This is an abbreviated version!
For detailed information about hydrogenase (acceptor), go to the full flat file.

Word Map on EC 1.12.99.6

Reaction

H2
+
acceptor
=
reduced acceptor

Synonyms

ECH, Ech hydrogenase, EchA, EchB, EchD, EchE, EchF, endo-hydrogenase, energy-converting hydrogenase, exo-hydrogenase, F420-reducing [NiFe] hydrogenase, factor420 hydrogenase, Fe-hydrogenase, Fe-only hydrogenase, ferredoxin hydrogenase, H2 producing hydrogenase [ambiguous], H2-sensing [NiFe] hydrogenase, Hfs, Hmd, HoxEFUYH type [NiFe] hydrogenase, HoxG, HupB, HupL, HupS, hupSLW, Hya, HyaA, HyaB, Hyb, HybA, HyC, HycE, Hyd X, Hyd-1, Hyd-2, Hyd-3, Hyd-4, HYD1, hydA, HydA1, HydA2, HydB, hydrogen dehydrogenase, hydrogen uptake hydrogenase, hydrogen-forming methylenetetrahydromethanopterin dehydrogenase, hydrogen-lyase, hydrogen-lyase [ambiguous], hydrogen:ferredoxin oxidoreductase, hydrogen:methylviologen oxidoreductase, hydrogenase (ferredoxin), hydrogenase 1, hydrogenase 2, hydrogenase 3, hydrogenase I (bidirectional), hydrogenase II, hydrogenase II (uptake), hydrogenase-1, hydrogenase-2, hydrogenase-Fe-S, hydrogenases Hyd-1, hydrogenases Hyd-2, hydrogenlyase [ambiguous], hyf, HyhL, HynSL, HynSL hydrogenase, Hyq, iron-sulfur-cluster-free hydrogenase, Mbh, membrane-bound [NiFe]-hydrogenase, methyl viologen-reducing hydrogenase, methylviologen hydrogenase, Ni-Fe hydrogenase, nickel-iron hydrogenase, NiFe hydrogenase, NiFe(Se) hydrogenase, NiFe-hydrogenase, NIFeSe-hydrogenase, regulatory hydrogenase, respiratory hydrogenase, SHI, uptake hydrogenase, uptake hydrogenase [ambiguous], uptake [NiFe] hydrogenase:, uptake [NiFe]-hydrogenase, [FeFe] hydrogenase, [FeFe]-H2ase, [FeFe]-hydrogenase, [FeFe]H2ase, [Fe] hydrogenase, [Fe]-hydA, [Fe]-hydrogenase, [Ni-Fe] hydrogenase, [NiFeSe]-hydrogenase, [NiFe] hydrogenase, [NiFe]-hydrogenase, [NiFe]-hydrogenase 2, [NiFe]-hydrogenase-2, [NiFe]H2ase, [NiFe]hydrogenase

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.99 With unknown physiological acceptors
                1.12.99.6 hydrogenase (acceptor)

Cloned

Cloned on EC 1.12.99.6 - hydrogenase (acceptor)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
an overexpression system is described that facilitates the determination of high-resolution crystal structures of HybOC and, hence, a prediction of the quaternary structure of the HybOCAB complex
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli JM109 cells
-
expression in Escherichia coli
expression in Escherichia coli as GST-tagged enzyme
-
expression of Strep-tagged enzyme
-
genes encoding the large (hydA) and small (hydB) subunits of the [FeFe] hydrogenase cloned in T7 promoter plasmids. Expression in Escherichia coli
-
heterologous expression and synthesis of [FeFe] hydrogenase in Clostridium acetobutylicum ATCC 824
-
heterologously expressed in Escherichia coli BL21(DE3) from vector pY71 N-His6-shydA1*
-
hydA1 and hydA2 expressed in Escherichia coli
-
HydADELTAEFG cloned into a pET Duet vector and modified for the presence of an N-terminal six-histidine tag. Expressed in Escherichia coli BL21(DE3) cells
-
mutant enzymes expressed in Escherichia coli
-
overexpressed in a non-tagged version using plasmid pLO6, harboring the complete MBH operon, in Ralstonia eutropha strain HF631
-
overproduced in Escherichia coli BL21(DE3) cells
-
variants expressed from epPCR and DNA shuffling plasmid pBS(Kan)HycE in Escherichia coli BW25113 hyaB hybC hycE DELTAkan
-