1.12.98.4: sulfhydrogenase

This is an abbreviated version!
For detailed information about sulfhydrogenase, go to the full flat file.

Word Map on EC 1.12.98.4

Reaction

H2
+
(sulfide)n
=
hydrogen sulfide
+
(sulfide)n-1

Synonyms

H-II, H2:sulfuroxidoreductase complex, hydrogenase II, NiFe sulfhydrogenase, polysulfide dehydrogenase, polysulfide reductase, reductase, sulfur, SH2ase, sulfhydrogenase, sulfur reductase, sulhydrogenase

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.98 With other, known, physiological acceptors
                1.12.98.4 sulfhydrogenase

Purification

Purification on EC 1.12.98.4 - sulfhydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
aerobic and anaerobic conditions
-
DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
enzyme complex
-
hydroxyapatite chromatography; purified with 2-octylglucoside, not perchlorate or urea
-
isolation and incorporation into liposomes
-
partial, solubilized with Triton X-100
-
purification of enzyme by solubilísation with sodium deoxycholate, in presence of ACA and glycerol
-
Q Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography
-