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1.12.2.1: cytochrome-c3 hydrogenase

This is an abbreviated version!
For detailed information about cytochrome-c3 hydrogenase, go to the full flat file.

Word Map on EC 1.12.2.1

Reaction

H2
+ 2 ferricytochrome c3 = 2 H+ + 2 ferrocytochrome c3

Synonyms

cytochrome c3 hydrogenase, cytochrome c3 reductase, cytochrome hydrogenase, cytochrome-c3 hydrogenase, Fe-only hydrogenase, H2:ferricytochrome c3 oxidoreductase, H2ase, hyd B, HydAB, hydrogenase, hydrogenase, cytochrome, Hyn-1B, Hyn1 hydrogenase, HynAB-1, HysAB, [FeFe] hydrogenase, [Fe] hydrogenase, [Fe]-hydrogenase, [NiFeSe] Hase, [NiFeSe] hydrogenase, [NiFe] Hase, [NiFe] hydrogenase, [NiFe] hydrogenase 1, [NiFe]-hydrogenase

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.2 With a cytochrome as acceptor
                1.12.2.1 cytochrome-c3 hydrogenase

Crystallization

Crystallization on EC 1.12.2.1 - cytochrome-c3 hydrogenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
X-ray diffraction structure determination and analysis of purified reduced cytochrome C3, which is associated with the enzyme, at 0.1 M Tris-HCl, pH 7.6, and 60% PEG 400, at 1.35-1.4 A resolution, comparison to the structure of oxidized cytochrome c3 at pH 4.0, overview, modeling
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crystallization by vapor diffusion method with polyethylene glycol or 2-methyl-2,4-pentanediol as precipitating agents. Seeding procedure is necessary to grow an X-ray grade crystal
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