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1.12.1.4: hydrogenase (NAD+, ferredoxin)

This is an abbreviated version!
For detailed information about hydrogenase (NAD+, ferredoxin), go to the full flat file.

Word Map on EC 1.12.1.4

Reaction

2 H2 +

NAD+
+ 2 oxidized ferredoxin = 5 H+ +
NADH
 + 2 reduced ferredoxin

Synonyms

bifurcating [FeFe] hydrogenase, electron-bifurcating ferredoxin- and NAD-dependent hydrogenase, Fe-only hydrogenase, HydABC, HydC, HydF, iron hydrogenase, NAD(P)+-linked [FeFe]-hydrogenase, [FeFe]-hydrogenase, [FeFe]-type hydrogenase

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.1 With NAD+ or NADP+ as acceptor
                1.12.1.4 hydrogenase (NAD+, ferredoxin)

Purification

Purification on EC 1.12.1.4 - hydrogenase (NAD+, ferredoxin)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration
-
nickel affinity column chromatography and Superdex 200 gel filtration
-
nickel affinity column chromatography, and Superose 12 gel filtration
-
streptactin column chromatography
-
under strictly anoxic conditions, the enzyme is inactivated in the presence of even trace amounts of O2
-