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C461A
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive
D456N
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive
D456S
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive
E14Q
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive
E14V
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive
G15A
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive
H396L
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site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain
H396Q
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site-directed mutagenesis, catalytic subunit HoxH allele, about 3.5fold reduced activity compared to the wild-type strain
H69Q
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site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain
H69Q/P390A
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site-directed mutagenesis, catalytic subunit HoxH alleles, reduced activity compared to the wild-type strain
I64A
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain
L118A
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site-directed mutagenesis, catalytic subunit HoxH allele, reduced activity compared to the wild-type strain
L118F
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow at O2 concentrations above 5%, about 4fold reduced activity compared to the wild-type strain
L118I
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site-directed mutagenesis, catalytic subunit HoxH allele, reduced activity compared to the wild-type strain
L394A
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site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain
L394N
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive
P390A
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site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain
R12L
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain
R12Q
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain
R391L
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive
R391Q
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive
R60Q
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site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain
S68V
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site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain
T415S
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site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain
T415V
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site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain
T415V/N415H
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site-directed mutagenesis, catalytic subunit HoxH alleles, O2-sensitive growth, highly reduced activity compared to the wild-type strain
I64M
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the mutation alters gas diffusion kinetics and improves O2 tolerance
I64M/L107M
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the mutant shows strongly reduced activity compared to the wild type enzyme
I64M/L107M/L112M
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the mutant shows strongly reduced activity compared to the wild type enzyme
I64M/L112M
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the mutant shows strongly reduced activity compared to the wild type enzyme
L107F
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the mutant shows reduced activity compared to the wild type enzyme
L107M/L112M
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the mutant shows reduced activity compared to the wild type enzyme
L112F
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the mutant shows strongly reduced activity compared to the wild type enzyme
L112M
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the mutant shows slightly reduced activity compared to the wild type enzyme
H16L
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site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain
H16L
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in the HoxH-mutant protein H16L, H2 oxidation is impaired, but H2 production occurrs via a stable Ni-C state (N(III)-(H-)-Fe(II))
additional information
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construction of a HoxI deletion mutant enzyme, which shows different activation behaviour than the wild-type, it is not activated by and does not react with NADPH
additional information
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construction of an inactive null mutant strain, mutant strain growth characteristics, overview
additional information
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construction of a HoxI deletion mutant enzyme, which shows different activation behaviour than the wild-type, it is not activated by and does not react with NADPH
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additional information
in-frame deletion of HoxE, deletion of HoxH
additional information
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in-frame deletion of HoxE, deletion of HoxH