1.12.1.2: hydrogen dehydrogenase

This is an abbreviated version!
For detailed information about hydrogen dehydrogenase, go to the full flat file.

Reaction

Synonyms

bidirectional hydrogenase, bidirectional Ni-Fe hydrogenase, bidirectional NiFe-hydrogenase, bidirectional [NiFe] hydrogenase, dehydrogenase, hydrogen, FeHyd, H2:NAD+ oxidoreductase, H2ase, Hox, Hox hydrogenase, Hox type hydrogenase, HoxE, HoxEFUYH, HoxF, HoxFUYHI2, HoxH, HoxS, HoxU, HoxY, hydrogen-evolving enzyme, hydrogenase, Hyn, iron-hydrogenase, NAD+-reducing hydrogenase, NAD+-reducing NiFe hydrogenase, NAD+-reducing [NiFe]-hydrogenase, NAD-dependent hydrogenase, NAD-reducing hydrogenase, NADH-dependent Fe-only hydrogenase, NiFe hydrogenase, NiFe-type reversible hydrogenase, SH, soluble hydrogenase, soluble [NiFe]-hydrogenase, [FeFe] hydrogenase, [FeFe]-hydrogenase, [NiFe] H2ase, [NiFe] hydrogenase, [NiFe]-hydrogenase

ECTree

     1 Oxidoreductases

         1.12 Acting on hydrogen as donor

             1.12.1 With NAD⁺ or NADP⁺ as acceptor

                1.12.1.2 hydrogen dehydrogenase

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Results	in table
23	Activating Compound
1071	AA Sequence
5	Application
2	CAS Registry Number
8	Cloned(Commentary)
63	Cofactor
1	Crystallization (Commentary)
1	General Information
6	General Stability
30	Inhibitors
2	Ki Value [mM]
38	KM Value [mM]
35	Localization
65	Metals/Ions
25	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
647	Organism
11	Oxidation Stability
4	Pathway
8	PDB ID
22	pH Optimum
3	pH Range
5	pH Stability
1	Posttranslational Modification
40	Protein Variants
18	Purification (Commentary)
6	Reaction
3	Reaction Type
116	Reference
12	Source Tissue
61	Specific Activity [micromol/min/mg]
5	Storage Stability
261	Substrates and Products (Substrate)
31	Subunits
104	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
4	Temperature Stability [°C]
21	Turnover Number [1/s]

Activating Compound

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Activating Compound on EC 1.12.1.2 - hydrogen dehydrogenase

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

FMN

3 entries

glutathione

Rhodococcus opacus

-

up to 0.1 mM: activation, above 0.1 mM: inactivation

439701

HypC

Thermococcus kodakarensis

-

[NiFe] hydrogenase maturation protein HypC catalyzes the insertion and cyanation of the iron center of [NiFe] hydogenase

688938

-

HypD

Thermococcus kodakarensis

-

[NiFe] hydrogenase maturation protein HypD catalyzes the insertion and cyanation of the iron center of [NiFe] hydogenase

688938

-

HypE

Thermococcus kodakarensis

-

[NiFe] hydrogenase maturation protein HypE catalyzes the insertion and cyanation of the iron center of [NiFe] hydogenase

688938

-

LexA protein

Synechocystis sp.

-

the transcription factor LexA contributes to enzyme expression

687403

-

Na2S2O4

Cupriavidus necator

-

1 mM, activity of initially inactive enzyme increases within 1 h to a stable level in the presence of 101 kPa H2

439696

NADH

3 entries

NADPH

2 entries

NH4+

Rhodococcus opacus

-

activates

439701

phosphate

Rhodococcus opacus

-

increase in NAD-reduction rate

439701

Sll0359 protein

Synechocystis sp.

-

the AbrB-like protein Sll0359 interacts specifically with the hox promoter region and works as an activator of hox gene expression

687403

-

SO42-

Rhodococcus opacus

-

activates

439701

[NiFe] hydrogenase maturation protein

Escherichia coli

-

maturation of the [NiFe] hydrogenase involves assembly of nonproteinaceous ligands on the large subunit by accessory proteins encoded by the hyp operon, HypE is an essential accessory protein and participates in the synthesis of two cyano groups found in the large subunit

687407

-

additional information

4 entries

-

FMN

Caldanaerobacter subterraneus subsp. tengcongensis

-

activates

656734

FMN

Cupriavidus necator

-

-

656298

FMN

Cupriavidus necator

P22319, P22320

H2 oxidation by subunits HoxHY is enhanced on addition of excess FMN

724910

NADH

Cupriavidus necator

-

-

439696

NADH

Cupriavidus necator

-

specific activation of the soluble oligomeric enzyme

655890

NADH

Cupriavidus necator

-

activates hexameric enzyme form, no activation of the tetrameric enzyme form

655890

NADPH

Cupriavidus necator

-

specific activation of the soluble oligomeric enzyme, the binding domain is located on subunit HoxI

655890

NADPH

Cupriavidus necator

-

activates hexameric enzyme form. Subunit HoxI provides a binding domain for NADPH

655890

additional information

Cupriavidus necator

-

at a redox potential of approx. -100 mV the inactive form of the enzyme is transformed into an active one

439705

-

additional information

Cupriavidus necator

-

enzyme activation mechanism, overview

655890

-

additional information

Synechocystis sp. PCC 6803

-

putative regulators of hox gene expression sll0359 is induced in light to a similar extent as hoxU, hoxY and hoxH. The transcriptional regulator lexA shows the opposite expression pattern than that of the hox genes, since it is induced in darkness by up to 3fold. When cells in microaerobic conditions are transferred to darkness the transcript levels of hoxE and hoxF are further increased

704766

-

additional information

Thermococcus kodakarensis

-

the hydrogenase maturation protein HypE catalyzes the synthesis of the CN ligand of the active-site iron of the [NiFe] hydrogenase using carbamoyl phosphate and ATP as substrates (HypE dehydrates the S-carbamoyl moiety by an ATP-dependent reaction to produce HypE-thiocyanate)

684181

-