1.11.1.B6: iodide peroxidase (vanadium-containing)
This is an abbreviated version!
For detailed information about iodide peroxidase (vanadium-containing), go to the full flat file.
Reaction
Synonyms
iodooperoxidase, iodoperoxidases PcI, iodoperoxidases PcII, V-containing-haloperoxidase, vanadium-dependent haloperoxidase, vanadium-dependent iodoperoxidase, vIPO, zobellia_1262
ECTree
Advanced search results
Application
Application on EC 1.11.1.B6 - iodide peroxidase (vanadium-containing)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
analysis
additional information
-
for iodoperoxidase activity measurement, the triiodide assay should only be used for qualitative applications. A quantitative spectrophotometric assay is based on the iodination of thymol blue but can only be used in the pH range 7-8
analysis
additional information
-
to detect activity on polyacrylamide gels or in cross sections of algal part, o-dianisidine may be used. The probes are first incubated in a buffered solution containing 100 mM KBr or 100 mM KI, and 10 mM o-dianisidine, and the staining is started by addition of 2 mM H2O2. The formed HOBr or HOI will oxidize the organic dye resulting in polymerization and formation of a brown precipitate