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1.11.1.B2: chloride peroxidase (vanadium-containing)

This is an abbreviated version!
For detailed information about chloride peroxidase (vanadium-containing), go to the full flat file.

Word Map on EC 1.11.1.B2

Reaction

RH
+
Cl-
 +
H2O2
 +
H+
=
RCl
 + 2 H2O

Synonyms

chloroperoxidase, CPO, Mcl24, More, NapH1, PPHY, V-containing-haloperoxidase, vanadium chloroperoxidase, vanadium haloperoxidase, vanadium-containing chloroperoxidase, vanadium-containing peroxidase, vanadium-dependent chloroperoxidase, vanadium-dependent haloperoxidase, vCPO, VHPO

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.B2 chloride peroxidase (vanadium-containing)

Cloned

Cloned on EC 1.11.1.B2 - chloride peroxidase (vanadium-containing)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
3000fold overexpression in Streptomyces aureofaciens Tü24-88
-
expressed in Escherichia coli
-
expressed in Saccharomyces cerevisiae
expression in Escherichia coli
expression in Saccharomyces cerevisiae
gene pphy, sequence comparisons, subcloning in Escherichia coli XL10-Gold cells, expression in Pichia pastoris strain X33, the recombinant enzyme is secreted
recombinant CPO and mutant enzymes S402A and F397H are overexpressed as apoenzyme in Saccharomyces cerevisiae
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-Gold (DE3)