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1.11.1.6: catalase

This is an abbreviated version!
For detailed information about catalase, go to the full flat file.

Word Map on EC 1.11.1.6

Reaction

2 H2O2 =

O2
+ 2 H2O

Synonyms

Ab-catalase, BNC, caperase, CAT, CAT-1, CAT-A, CAT-P, Cat1.4, CatA, catalase, catalase A, catalase C, catalase form III, catalase P, catalase-1, catalase-A, catalase-peroxidase, catalase-phenol oxidase, CatB, CATC, CatF, CatG, CatP, CATPO, CcmC, CP, equilase, H2O2:H2O2 oxidoreductase, haem catalase, HPI-A, HPI-B, HPII, HTHP, hydrogen peroxide oxidoreductase, KAT, Kat E catalase, KatA, KatB, KatC, KatP, KpA, manganese catalase, More, optidase, PktA, polyethylene glycol-catalase, tyrosine-coordinated heme protein, VktA

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.6 catalase

Cloned

Cloned on EC 1.11.1.6 - catalase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analyis
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DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression during hypoxia and reoxygenation, 1 h after hypoxia, overview
DNA and amino acid sequence determination and analysis, quantitative real-time RT-PCR expression analysis, phylogenetic analysis
DNA andminoa cid sequence determination andd analysis, full length cDNA sequence, RT-PCR expression analysis
expressed in Escherichia coli Rosetta (DE3)
expressed in in MCF-7, Caco-2, and Hct-116 cells, catalase overexpression is associated with increased sensitivity to tisse necrosis facor-alpha
-
expression analysis in strain PAO1 of biofilms of Pseudomonas aeruginosa
-
expression as His-tagged enzyme in constructed enzyme-deficient Bacillus subtilis strain BLF03, overexpression of wild-type and His-tagged enzyme in Enterococcus faecalis strain V583 utilizing an Escherichia coli-Enterococcus faecalis-shuttle plasmid
-
expression in Bacillus subtilis
expression in catalase-deficient Escherichia coli streain UM2
expression in Escherichia coli
expression in Escherichia coli as fusion protein with maltose binding protein
expression in Escherichia coli, 100-fold increase in active enzyme in presence of 1 mM Mn2+ in the induction medium
expression in Pichia pastoris
expression in Sf9 cell
expression of mutant M244A in Haloferax denitrificans
-
expression of soluble and inactive His-tagged catalase-A in Escherichia coli strain BL21(DE3) in inclusion bodies. Specific requirement of a thioredoxin fusion partner, the involvement of trigger factor protein and the low temperature treatment during induction period for synthesis of completely solubilized rice plant catalase-A in recombinant Escherichia coli. The bacteria require the supplementation of delta-aminolevulinic acid to produce bio-active recombinant rice catalase-A
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 Star (DE3)
-
expression of wild-type and mutant His-tagged Cat1.4 isozymes in Escherichia coli strain BL21 (DE3)
-
gene ctaDII, which codes for subunit I of CcO, expression in Escherichia coli strain DH5alpha, the recombinant enzyme shows reduced catalase activity and thermal stability, overview. Expression of subunit I in the wild-type strain increases the catalase activity by 20fold. Overproduction of SU I might impair the correct insertion of heme a3 and CuB because of a deficiency in metal inserting chaperones. An altered distance between heme a3 and CuB and variations in protein structure are possible reasons for the observed increased catalase activity. Co-expression of chaperone-encoding genes genes ctaG and surf1c reduces the catalase activity of the organism, thermal stability is altered about aging, overview
gene expression analysis during flowering and seed development
-
gene katA, DNA and amino acid sequence determination, comparison, and analysis
gene katA, functional expression in lipodopteran insect cells Sf9 and Sf21 from Spodoptera fruiperda, and High5 from Trichoplusia ni via baculovirus-infection, expression analysis, the amount of apoptotic body formation decreased in transfected cells
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gene katA, screening of a genetic library, and DNA sequence determination and analysis, phylogenetic analysis, temperature-sensitive expression in enzyme-deficient strain Escherichia coli at 28°C, 200-300fold overexpression in a Xanthomonas campestris mutant strain XpHR
gene katE, expression as fusion enzyme with aldehyde deformylating oxygenase, ADO, from Prochlorococcus marinus
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gene katP, expression of wild-type and mutant enzymes in strain XL-1 Blue and as His-tagged proteins in BL21(DE3)
-
gene katPc, DNA and amino acid sequence determination and analysis
gene PbcatP, DNA and amino acid sequence determination and analysis, expression analysis, phylogenetic analysis
katB, expression in Escherichia coli
-
when the wild-type and mutant (M129V/E293G) genes are overexpressed in Escherichia coli, unmodified or six-His-tagged proteins of the expected size are overproduced as inactive proteins. To overcome this problem, the Mn2+-dependent catalase genes are overexpressed directly in Thermus thermophilus under the control of the Pnar promoter. This promoter belongs to a respiratory nitrate reductase from of Thermus thermophilus HB8, whose transcription is activated by the combined action of nitrate and anoxia. Upon induction in Thermus thermophilus HB8, a 20fold to 30fold increase in catalase specific activity is observed, whereas a 90fold to 110fold increase is detected when the laboratory strain Thermus thermophilus HB27::nar