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1.11.1.5: cytochrome-c peroxidase

This is an abbreviated version!
For detailed information about cytochrome-c peroxidase, go to the full flat file.

Word Map on EC 1.11.1.5

Reaction

2 ferrocytochrome c +

H2O2
+ 2 H+ =
2 ferricytochrome c
 + 2 H2O

Synonyms

apocytochrome c peroxidase, BCcP, CCP, CCP1, CcpA, Cjj0382, CytC, cytochrome c iso-1, cytochrome c peroxidase, cytochrome c-551 peroxidase, cytochrome c-H2O oxidoreductase, cytochrome peroxidase, di-heme cytochrome c peroxidase, diheme cytochrome c peroxidase, diheme cytochrome c5 peroxidase CcpA, DocA, LmP, MacA, mesocytochrome c peroxidase azide, mesocytochrome c peroxidase cyanate, mesocytochrome c peroxidase cyanide, peroxidase, cytochrome c, Psa CcP

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.5 cytochrome-c peroxidase

Cloned

Cloned on EC 1.11.1.5 - cytochrome-c peroxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli JM109 (DE3) cells
expressed in Escherichia coli strain BL21(DE3)
-
expression in Escherichia coli
expression in Escherichia coli JM109(DE3) with pETCCP coexpressed with pEC86
expression of maltose-binding-protein-fusion and tag-free CcP in Escherichia coli strain JM 109, the presence of the MBP tag affects the availability of certain binding sites, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
expression of V197C/C128A mutant in Escherichia coli
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
functional expression of deuterated and soluble His-tagged Ccp in Escherichia coli strain BL21(DE3). Introduction of a His-tag at either protein terminus dramatically increases its solubility, allowing preparation of concentrated, stable CcP samples. The engineered His tags neither perturb the structure of the enzyme nor alter the heme environment or its reactivity toward known ligands
-
gene ccpA, expression of His-tagged CcpA in Escherichia coli strain AS457
-
gene macA, expression as N-terminally Strep-tagged protein in Escherichia coli strain BL21(DE3)
mutants expressed in Escherichia coli
-
overexpression in Escherichia coli
-
overexpression in Escherichia coli in deuterated medium
overexpression of modified enzyme in Escherichia coli
recombinant expression of wild-type and mutant enzymes
-