1.11.1.23: (S)-2-hydroxypropylphosphonic acid epoxidase

This is an abbreviated version!
For detailed information about (S)-2-hydroxypropylphosphonic acid epoxidase, go to the full flat file.

Word Map on EC 1.11.1.23

Reaction

(S)-2-hydroxypropylphosphonate
+
H2O2
=
(1R,2S)-1,2-epoxypropylphosphonate
+ 2 H2O

Synonyms

HPP epoxidase, 2-hydroxypropylphosphonic acid epoxidase, HppE, Ps-HppE, fom4, (S)-2-hydroxypropylphosphonic acid epoxidase, fosfomycin-producing epoxidase, (S-HPP) epoxidase, (S)-2-HPP epoxidase

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.23 (S)-2-hydroxypropylphosphonic acid epoxidase

Engineering

Engineering on EC 1.11.1.23 - (S)-2-hydroxypropylphosphonic acid epoxidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H180A
complete loss of enzymatic activity in vivo and in vitro, 5.8% of the active site iron as compared to wild-type enzyme, no self-hydroxylation at Tyr105
E142A
complete loss of enzymatic activity in vivo and in vitro, 2.1% of the active site iron as compared to wild-type enzyme, no self-hydroxylation at Tyr105
Y103F
mutant maintains 60% of the activity of the wild-type enzyme
H138A
inactive in vivo, but in vitro it retains 27% of the active site iron and nearly 20% of the wild-type activity, limited self-hydroxylation (at Tyr105)
Y105F
mutant enzyme is inactive
Y102F
mutant maintains 60% of the activity of the wild-type enzyme