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1.11.1.21: catalase-peroxidase

This is an abbreviated version!
For detailed information about catalase-peroxidase, go to the full flat file.

Word Map on EC 1.11.1.21

Reaction

2 H2O2 =

O2
+ 2 H2O

Synonyms

AfKatG, BW16_04845, CAT, CAT-2, catalase -peroxidase KatG, catalase peroxidase, catalase-peroxidase, catalase/peroxidase, CP 2, CP01, CP02, CPX, CthediskatG, EC 1.11.1.7, FeSOD A, FvCP01, FvCP02, FVEG_10866, FVEG_12888, HCP, hemoprotein b-590, HPI, hydroperoxidase I, KatG, KatG1, KatG2, KatP, katX2, KpCP, PCP, Rv1908c

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.21 catalase-peroxidase

Specific Activity

Specific Activity on EC 1.11.1.21 - catalase-peroxidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00904
-
specific peroxidase activity, using isoniazid as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C
0.0098
-
specific peroxidase activity, using isoniazid as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C
0.16
-
catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C
0.18
-
substrate o-dianisidine, mutant H290Q
0.208
-
specific peroxidase activity, using NADH as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C
0.212
-
specific peroxidase activity, using NADH as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C
0.25
-
catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C
0.264
-
specific peroxidase activity, using NADPH as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C
0.272
-
specific peroxidase activity, using NADPH as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C
0.45
peroxidase activity from crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
0.5
-
substrate guajacol, mutant P151A
0.55
-
peroxidase activity, using guaiacol as substrate, mutant enzyme N153A, in 50 mM phosphate buffer, pH 7.0, and 30°C
0.61
-
crude enzyme, peroxidase activity, in 67 mM phosphate buffer, pH 7.0, at 25°C
0.8
-
substrate o-dianisidine, mutant W341A
1
-
peroxidase specific activity, using o-dianisidine as substrate, at pH 5.0 and 25°C
1.7
-
after 2.79fold purification, peroxidase activity, in 67 mM phosphate buffer, pH 7.0, at 25°C
1.8
-
substrate o-dianisidine, mutant D402N
100.7
-
specific peroxidase activity, using 4-phenylendiamine as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C
104.3
-
after 29.6fold purification, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C
1145
-
after 47.3fold purification, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C
12.5
-
substrate pyrogallol, mutant D152N
13.6
-
substrate pyrogallol, mutant D152S
1300
-
peroxidase activity, mutant enzyme W105L, at pH 7.0 and 37°C
14.9
purified recombinant intein-tagged enzyme, pH 5.5, 25°C, peroxidase activity
150
-
peroxidase activity using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 5.4 and 25°C
16
-
peroxidase activity using 3,3'-diaminobenzidine as substrate, at pH 5.4 and 25°C
168
-
unpurified cytosolic fraction, peroxidase activity, in 50 mM Tris-HCl buffer (pH 8.5), at 30°C
18
-
peroxidase activity, mutant enzyme H106Y, at pH 7.0 and 37°C
19
-
catalase activity, mutant enzyme R102L, at pH 7.0 and 37°C
2
-
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 5.0 and 25°C
2.1
-
substrate o-dianisidine, mutant D402E
2.12
-
specific peroxidase activity, using pyrogallol as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C
2.29
-
specific peroxidase activity, using pyrogallol as substrate, purified mutant enzyme R463L, in 50 mM Na2HPO4, pH 5.5, 25°C
22.8
-
specific peroxidase activity, using o-dianisidine as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C
226
-
specific catalase activity, crude extract, in 50 mM Na2HPO4, pH 7.0, 25°C
23.3
peroxidase activity after 3.17fold purification, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
2375
catalase activity after heat treatment of the crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 6.0, at 70°C
24.2
-
crude extract, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C
2420
-
specific catalase activity, after 10.7fold purification, in 50 mM Na2HPO4, pH 7.0, 25°C
25.9
-
specific peroxidase activity, using o-dianisidine as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C
250
-
cell extract, at 25°C, pH 6.4
28
-
peroxidase activity using 3,3'-dimethoxybenzidine as substrate, at pH 5.4 and 25°C
2960
-
purified enzyme, at 25°C, pH 6.4
3.4
-
crude extract, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C
3.8
-
peroxidase activity, using o-dianisidine as substrate, wild type enzyme, in 50 mM phosphate buffer, pH 7.0, and 30°C
3.9
-
crude extract, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C
3120
-
catalase specific activity, at pH 7.0 and 37°C
316
-
unpurified cytosolic fraction, catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C
35
-
catalase activity, mutant enzyme R102K, at pH 7.0 and 37°C
35000
-
after 110.7fold purification, catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C
3630
-
catalase specific activity, at pH 7.0 and 37°C
368
-
crude enzyme, catalase activity, in 67 mM phosphate buffer, pH 7.0, at 30°C
4.3
-
peroxidase activity, using o-dianisidine as substrate, mutant enzyme N153D, in 50 mM phosphate buffer, pH 7.0, and 30°C
4.4
-
crude extract, in 67 mM phosphate buffer (pH 7.0), at 25°C
4.8
-
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.5 and 25°C
43
-
crude enzyme, in 100 mM potassium phosphate buffer (pH 6.8), at 30°C
43.2
-
after 12.7fold purification, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C
4450
-
catalase specific activity, at pH 7.0 and 37°C
4500
-
catalase activity, in phosphate buffer, pH 7.5, at 25°C
4830
-
catalase specific activity, at pH 7.0 and 37°C
5
-
crude extract, catalatic activity, at 20°C and pH 7.5, in the presence of 1.5 M NaC1
5.5
-
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.3 and 25°C
5280
-
catalase specific activity, at pH 7.0 and 37°C
533
-
after 214fold purification, in 50 mM phosphate buffer, pH 7.0, at 25°C
5420
-
catalase specific activity, at pH 7.0 and 37°C
55784
substrate H2O2, pH 7.0, 37°C
560
-
after 13fold purification, in 100 mM potassium phosphate buffer (pH 6.8), at 30°C
6.1
-
substrate o-dianisidine, mutant W341F
6.4
-
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.3 and 25°C
6.6
-
substrate pyrogallol, wild-type enzyme
63
-
peroxidase activity, mutant enzyme R102C, at pH 7.0 and 37°C
660
-
peroxidase activity, wild type enzyme, at pH 7.0 and 37°C
6922
catalase activity after 2.91fold purification, in 50 mM potassium phosphate/sodium citrate buffer, pH 6.0, at 70°C
7.3
-
substrate pyrogallol, mutant D152W
743
-
peroxidase activity, pH 7.0, 37°C
7512
-
after 44.7fold purification, peroxidase activity, in 50 mM Tris-HCl buffer (pH 8.5), at 30°C
77
-
peroxidase activity using 2,6-dimethoxyphenol as substrate, at pH 5.4 and 25°C
785
-
after 178fold purification, in 67 mM phosphate buffer (pH 7.0), at 25°C
8.3
-
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.3 and 25°C
8.8
-
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.8 and 25°C
818
-
catalase activity, pH 7.0, 37°C
851
-
after 170fold purification, catalatic activity, at 20°C and pH 7.5, in the presence of 1.5 M NaC1
983
-
after 2.67fold purification, catalase activity, in 67 mM phosphate buffer, pH 7.0, at 30°C
99.4
-
specific peroxidase activity, using 4-phenylendiamine as substrate, purified wild type enzyme, in 50 mM Na2HPO4, pH 5.5, 25°C
additional information
-
isoniazid activation activities of wild-type and mutant enzymes in absence or presence of Mn2+, overview