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1.11.1.21: catalase-peroxidase

This is an abbreviated version!
For detailed information about catalase-peroxidase, go to the full flat file.

Word Map on EC 1.11.1.21

Reaction

2 H2O2 =

O2
+ 2 H2O

Synonyms

AfKatG, BW16_04845, CAT, CAT-2, catalase -peroxidase KatG, catalase peroxidase, catalase-peroxidase, catalase/peroxidase, CP 2, CP01, CP02, CPX, CthediskatG, EC 1.11.1.7, FeSOD A, FvCP01, FvCP02, FVEG_10866, FVEG_12888, HCP, hemoprotein b-590, HPI, hydroperoxidase I, KatG, KatG1, KatG2, KatP, katX2, KpCP, PCP, Rv1908c

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.21 catalase-peroxidase

Purification

Purification on EC 1.11.1.21 - catalase-peroxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and DEAE-cellulose column chromatography
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephacryl S300 gel filtration
-
ammonium sulfate precipitation, Fractogel EMD 650(s) DEAE column chromatography, phenyl-Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S300 gel filtration
-
ammonium sulfate precipitation, Mono Q column chromatography, DEAE-Sephacel gel filtration, and Superdex S200 gel filtration
-
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Q-Sepharose column chromatography, hydroxyapatite column chromatography, and Superdex PG-200 gel filtration
-
ammonium sulfate precipitation, Sepharose 6B column chromatography, DEAE-Sephacel column chromatography, Sephadex G-25 gel filtration, and hydroxylapatite column chromatography
-
chelating Sepharose column chromatography, and hydroxyapatite column chromatography
DEAE-Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
-
DEAE-Sepharose column chromatography, Sephacryl S300 gel filtration, and MonoQ HR5/5 column chromatography
-
DEAE-Sepharose column chromatography, Superdex 200 gel filtration, and Resource Q column chromatography
HisPur Cobalt column chromatography
-
HisTrap column chromatography
Macrosphere GPC 300 column chromatography, hydroxylapatite column chromatography, and Sephadex G-150 gel filtration
-
Ni-NTA column chromatography
Ni-NTA column chromatography and phenyl-Sepharose column chromatography
-
Ni-NTA column chromatography, gel filtration
-
Ni-Sepharose column chromatography, Superdex 200 gel filtration
phenyl-Sepharose column chromatography, Sepharose column chromatography, and DEAE-cellulose chromatography
-
Q Sepharose column chromatography and Mono Q column chromatography
Q-Sepharose column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
Q-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography
-
Q-Sepharose column chromatography, Superdex 200 gel filtration, Mono Q column chromatography, and phenyl-Superose column chromatography
-
recombinant N-terminally His6-tagged enzyme 5.1fold (peroxidase activity) from Escherichia coli strain M15/pREP4 by nickel affinity chromatography, ultrafiltration, and gel filtration. Recombinant C-terminally intein-tagged enzyme 22.5fold from Escherichia coli by chitin affinity chromatography and treatment thereafter with a high concentration of DTT to excise the intein
recombinant protein
recombinant wild-type and mutant enzyme from strain PCC 6803
-
recombinant wild-type and mutant enzymes from Escherichia coli strain UM262, 50-90% homogeneity
-
Sepharose column chromatography, DEAE cellulose column chromatography, dialysis, hydroxyapatite column chromatography, and ACA 44 gel filtration
-
Zn2+-chelate affinity chromatography and phenyl-Sepharose column chromatography
-