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1.11.1.16: versatile peroxidase

This is an abbreviated version!
For detailed information about versatile peroxidase, go to the full flat file.

Word Map on EC 1.11.1.16

Reaction

1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol
+
H2O2
=
4-hydroxy-3-methoxybenzaldehyde
+
2-methoxyphenol
+
glycolaldehyde
+
H2O

Synonyms

B-type dye-decolorizing peroxidase, bacterial lignin peroxidase, DypB, manganese peroxidase 4, Mb peroxidase, metMb peroxidase, Mnp4, More, myoglobin, R1B4, versatile peroxidase, versatile peroxidase MnP2, versatile peroxidase VPL2 precursor, VP1, Vpl2, VPS1

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.16 versatile peroxidase

Purification

Purification on EC 1.11.1.16 - versatile peroxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, HiTrap Q column chromatography, and Mono Q column chromatography
-
DEAE FF' column chromatography and Superdex 200 gel filtration
extraction and purification of Aga2-VP fusion enzyme proteins from Saccharomyces cerevisiae strain EBY100 cell walls by dialysis, and ultrafiltration, followed by ion exchange chromatography
HiTrap Sepharose column chromatography
-
recombinant His-tagged N246A mutant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant protein
recombinant refolded His-tagged enzyme VP1 from Escherichia coli BL21(DE3) by nickel affinity chromatography
-
resource-Q chromatography
Resource-Q column chromatography