1.10.5.1: ribosyldihydronicotinamide dehydrogenase (quinone)

This is an abbreviated version!
For detailed information about ribosyldihydronicotinamide dehydrogenase (quinone), go to the full flat file.

Word Map on EC 1.10.5.1

Reaction

1-(beta-D-ribofuranosyl)-1,4-dihydronicotinamide
+
a quinone
=
1-(beta-D-ribofuranosyl)nicotinamide
+
a quinol

Synonyms

bQR2, dihydronicotinamide riboside:quinone oxidoreductase, dihydronicotinamide riboside:quinone oxidoreductase 2, dihydronicotinamide riboside:quinone reductase 2, EC 1.10.99.2, melatonin-binding site MT3, MT3, MT3/NQO2, N-ribosyldihydronicotinamide dehydrogenase (quinone), N-ribosyldihydronicotinamide:quinone oxidoreductase 2, NQO2, NRH-oxidizing enzyme, NRH:QR2, NRH:quinone oxidoreductase, NRH:quinone oxidoreductase 2, NRH:quinone oxireductase 2, NRH:quinone reductase 2, QR2, quinone oxidoreductase 2, quinone reductase 2, quinone reductase type 2

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.5 With a quinone or related compound as acceptor
                1.10.5.1 ribosyldihydronicotinamide dehydrogenase (quinone)

Crystallization

Crystallization on EC 1.10.5.1 - ribosyldihydronicotinamide dehydrogenase (quinone)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2.1 A resolution
crystal structure of the enzyme in complex with resveratrol. Crystals of the native QR2 and QR2-resveratrol complex all belong to the P2(1)2(1)2(1) space group
-
purified NQO2 in complex with primaquine and chloroquine, hanging drop vapor diffusion method, against reservoirs containing 0.1 M HEPES, pH 7.5, and 1.3-2.0 M (NH4)2SO4, crystals of NQO2-CQ are soaked in 0.001 ml of reducing-soak solution consisting of 0.1 M HEPES, pH 7.5, 2.0 M (NH4)2SO4, 10 mM 1-(3-sulfonatopropyl)-3-carbamoyl-1,4-dihydropyrimidine and 1 mM chloroquine, X-ray diffraction structure determination and analysis at 1.2-1.4 A resolution
-
purified recombinant enzyme, hanging-drop, vapor-diffusion method, mixing of 0.001 ml of 4 mg/ml protein solution with 0.001 ml of reservoir solution containing 1.3-1.7 M ammonium sulfate, 0.1 M Bis-Tris buffer, pH 6.0-7.0, 0.1 M NaCl, 5 mM DTT, 0.012 mM FAD, and resveratrol, complexed with different inhibitors, X-ray diffraction structure determination and analysis at resolutions of 1.40-1.63 A
-
the crystal structure of melatonin and 2-iodomelatonin in complex with quinone reductase 2 provide a detailed description of the enzyme active site that can now be utilized in the design of new and potent inhibitors of the enzyme, hanging-drop vapour-diffusion method
-
X-ray crystal structure of NQO2 bound to imatinib to 1.75 A resolution. The X-ray structure provides an explanation for the binding specificity of NQO2 for imatinib and nilotinib, as well as for the effects of mutation of the reported phosphorylation sites on NQO2
-
X-ray crystallography studies of QR2 in complex with 6-methoxy-9-methyl-[1,3]dioxolo[4,5-h]quinolin-8(9H)-one
-
crystal structure of quinone reductase 2 in complexes with dopamine and adrenochrome
-