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1.10.3.3: L-ascorbate oxidase

This is an abbreviated version!
For detailed information about L-ascorbate oxidase, go to the full flat file.

Word Map on EC 1.10.3.3

Reaction

4 L-ascorbate +

O2
= 4 monodehydroascorbate + 2 H2O

Synonyms

AA oxidase, AA-ox, AAO, AAO1, AAO2, AAO3, Aao4, Af_AO1, AO1, AO4, AOase, ASC oxidase, ascorbase, ascorbate dehydrogenase, ascorbate oxidase, ascorbic acid oxidase, ascorbic oxidase, ASOM, L-ascorbate:O2 oxidoreductase, L-ascorbic acid oxidase, oxidase, ascorbate

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.3 L-ascorbate oxidase

Storage Stability

Storage Stability on EC 1.10.3.3 - L-ascorbate oxidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, purified commercial enzymes, 10 mM HEPES-NaOH, pH 8.0, and 60% sucrose, comparison of the stability of the different enzyme batches, overview
Cucurbita sp.
-
12°C, at least 30 d, no loss of activity
Cucurbita pepo medullosa
-
4°C, 10 months, no loss in activity
Cucurbita pepo medullosa
-
4°C, 2 months, 20% loss of activity
Cucurbita pepo medullosa
-
4°C, ascorbate oxidase immobilized onto egg shell membrane through glutaraldehyde coupling in 0.1 M sodium phosphate EDTA buffer pH 5.6, 120 days, 50% loss of activity
-
4°C, concentrated solution, 3 months, 20% loss of activity
Cucurbita pepo medullosa
-