1.10.3.3: L-ascorbate oxidase
This is an abbreviated version!
For detailed information about L-ascorbate oxidase, go to the full flat file.
Word Map on EC 1.10.3.3
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1.10.3.3
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copper
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dehydroascorbic
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electrode
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laccase
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oxidases
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ceruloplasmin
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zucchini
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cucurbita
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cucumber
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amperometric
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trinuclear
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apoplastic
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monodehydroascorbate
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squash
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multi-copper
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ascorbate-dependent
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copper-containing
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plastocyanin
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pumpkin
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azurins
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ascorbyl
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ascorbate-glutathione
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myrothecium
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vernicifera
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ferroxidase
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agriculture
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medicine
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analysis
- 1.10.3.3
- copper
-
dehydroascorbic
-
electrode
- laccase
- oxidases
- ceruloplasmin
- zucchini
- cucurbita
- cucumber
-
amperometric
-
trinuclear
- apoplastic
- monodehydroascorbate
- squash
-
multi-copper
-
ascorbate-dependent
-
copper-containing
- plastocyanin
- pumpkin
- azurins
-
ascorbyl
-
ascorbate-glutathione
- myrothecium
- vernicifera
- ferroxidase
- agriculture
- medicine
- analysis
Reaction
4 L-ascorbate + = 4 monodehydroascorbate + 2 H2O
Synonyms
AA oxidase, AA-ox, AAO, AAO1, AAO2, AAO3, Aao4, Af_AO1, AO1, AO4, AOase, ASC oxidase, ascorbase, ascorbate dehydrogenase, ascorbate oxidase, ascorbic acid oxidase, ascorbic oxidase, ASOM, L-ascorbate:O2 oxidoreductase, L-ascorbic acid oxidase, oxidase, ascorbate
ECTree
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General Information
General Information on EC 1.10.3.3 - L-ascorbate oxidase
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evolution
malfunction
metabolism
physiological function
additional information
evolution
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AAO belongs to the large family of multicopper oxidases. AAO labelling in the cytosol, walls and chloroplasts of Chaetomorpha linum cells, whereas in angiosperms this enzyme is almost exclusively confined to the extracellular matrix, possible function of AAO in adaptation and functional evolution of its cellular location, overview
evolution
phylogenetic tree of plant ascorbate oxidases, overview
a diminution in ascorbate oxidase activity affects carbon allocation and improves yield in tomato under water deficit, overview. RNAi knockout plants show increased fruit yield under three conditions where assimilate became limiting for wild-type plants: when fruit trusses are left unpruned, when leaves are removed or when water supply is limited. Several alterations in the transgenic lines can contribute to the improved yield and favour transport of assimilate from leaves to fruits in the ascorbate oxidase lines. RNAi lines show altered sink strength
malfunction
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a diminution in ascorbate oxidase activity affects carbon allocation and improves yield in tomato under water deficit, overview. RNAi knockout plants show increased fruit yield under three conditions where assimilate became limiting for wild-type plants: when fruit trusses are left unpruned, when leaves are removed or when water supply is limited. Several alterations in the transgenic lines can contribute to the improved yield and favour transport of assimilate from leaves to fruits in the ascorbate oxidase lines. RNAi lines show altered sink strength
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stability of total vitamin C in situ was strongly dependent on the plant maturity stage and the processing conditions applied
metabolism
Cucurbita sp.
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the enzyme is involved in catalyzing the oxidation of apoplastic ascorbic acid to dehydroascorbic acid
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AAO plays a major role in the initial phase (oxidation of L-ascorbic acid to dehydroascorbic acid) of vitamin C degradation in broccoli
physiological function
ascorbate oxidase is a blue multicopper oxidase that catalyses the four-electron reduction of dioxygen to water with concomitant one-electron oxidation of the reducing organic substrate
physiological function
ascorbate oxidase catalyzes the oxidation of ascorbate to yield water. Enzyme overexpressing plants are prone to ozone and salt stresses, whereas lower expression apparently confers resistance to unfavorable environmental conditions. High enzme expression is viewed as a possible strategy to down-regulate oxygen diffusion in root nodules, and a component of arbuscular mycorrhizal symbiosis. Key role of this gene/enzyme in both nodule and arbuscular mycorrhizal development and functioning. The enzyme might be part of the general mechanism regulating cell wall architecture
physiological function
ascorbate oxidase is an apoplastic enzyme, which controls the redox state of the apoplastic ascorbate pool, effects of ascorbate oxidase on the apoplastic redox state, overview
physiological function
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ascorbate/dehydroascorbate play important roles in plant growth
physiological function
the enzyme is involved in antioxidant and redox regulation, overview. The protein catalyzes a complex reaction, with the safe reduction of molecular oxygen into water, without the release of reactive oxygen species, like hydrogen peroxide, superoxide anion, which result from partial O2 reduction
physiological function
the enzyme is involved in antioxidant and redox regulation, overview. The protein catalyzes a complex reaction, with the safe reduction of molecular oxygen into water, without the release of reactive oxygen species, like hydrogen peroxide, superoxide anion, which result from partial O2 reduction. Involvement of the enzyme in plant-microbe interaction
physiological function
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ascorbate oxidase and viral movement protein interaction helps in early viral movement of Cucumber mosaic virus
physiological function
the enzyme plays an important role in fiber cell elongation and may promote cell growth by generating the oxidation of apoplasts, via the auxin-mediated signaling pathway
physiological function
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ascorbate oxidase is an apoplastic enzyme, which controls the redox state of the apoplastic ascorbate pool, effects of ascorbate oxidase on the apoplastic redox state, overview
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proposed catalytic model, structure-function relationship, overview. Intramolecular electron transfer from the type-l copper centre to the trinuclear copper centre
additional information
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partially folded monomeric species might populate the energy landscape of the enzyme. The overall AAO stability is crucially controlled by a few quaternary interactions at the subunits' interface
additional information
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a rapid and sensitive ratiometric fluorescence sensing method for the detection of ascorbic acid and ascorbate oxidase is developed