1.10.3.3: L-ascorbate oxidase
This is an abbreviated version!
For detailed information about L-ascorbate oxidase, go to the full flat file.
Word Map on EC 1.10.3.3
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1.10.3.3
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copper
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dehydroascorbic
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electrode
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laccase
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oxidases
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ceruloplasmin
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zucchini
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cucurbita
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cucumber
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amperometric
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trinuclear
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apoplastic
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monodehydroascorbate
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squash
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multi-copper
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ascorbate-dependent
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copper-containing
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plastocyanin
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pumpkin
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azurins
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ascorbyl
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ascorbate-glutathione
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myrothecium
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vernicifera
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ferroxidase
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agriculture
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medicine
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analysis
- 1.10.3.3
- copper
-
dehydroascorbic
-
electrode
- laccase
- oxidases
- ceruloplasmin
- zucchini
- cucurbita
- cucumber
-
amperometric
-
trinuclear
- apoplastic
- monodehydroascorbate
- squash
-
multi-copper
-
ascorbate-dependent
-
copper-containing
- plastocyanin
- pumpkin
- azurins
-
ascorbyl
-
ascorbate-glutathione
- myrothecium
- vernicifera
- ferroxidase
- agriculture
- medicine
- analysis
Reaction
4 L-ascorbate + = 4 monodehydroascorbate + 2 H2O
Synonyms
AA oxidase, AA-ox, AAO, AAO1, AAO2, AAO3, Aao4, Af_AO1, AO1, AO4, AOase, ASC oxidase, ascorbase, ascorbate dehydrogenase, ascorbate oxidase, ascorbic acid oxidase, ascorbic oxidase, ASOM, L-ascorbate:O2 oxidoreductase, L-ascorbic acid oxidase, oxidase, ascorbate
ECTree
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Application
Application on EC 1.10.3.3 - L-ascorbate oxidase
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agriculture
analysis
food industry
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expression of enzyme gene in sense and antisense orientation, no significant differences in phenotype except for a delay in flowering time in antisense palnts. At high salinity, increase in percentage of germination, photosynthetic activity and seed yield in antisense plants. Sense plants show a very low redox state of apoplastic ascorbate and increased hydrogen peroxide contents in symplastic and apoplastic spaces
agriculture
expression of enzyme in sense- and antisense-orientations, enhanced enzyme activity oxidizes the apoplastic ascorbate pool, decreased enzyme activity increases the amount of ascorbate compared with dehydroascorbate. In sense and antisense plants, enzyme transcript levels are no longer subject to light/dark regulation. Relationship between enzyme activity and plant height and biomass
agriculture
up to 380fold increase in enzyme activity of leaf of transgenic plants, no change in total ascorbate content of apoplast, but redox state of ascorbate is reduced to below the threshold while that of glutathione is increased. Overexpressing plants show substantial increase in foliar injury and greater decline in CO2 assimilation upon exposure to ozone
agriculture
Cucurbita sp.
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when sprayed on sugar beet the enzyme works as an effective systemic defense priming agent against cyst nematode infection by Heterodera schachtii, through activation of multiple basal plant defense pathways
Cucurbita sp.
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quantitative determination of ascorbic acid with enzyme elctrode containing enzyme immobilized on nylon membrane
analysis
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rapid and simple determination of L-ascorbate in food with immobilized enzyme
analysis
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the enzyme is required in NO measurements to eliminates the interference of ascorbic acid and/or dehydroascorbic acid in the assays, overview
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the food enzyme is intended to be used in baking processes and cereal-based processes. It does not raise safety concerns under the intended conditions of use
food industry
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the food enzyme is intended to be used in baking processes and cereal-based processes. It does not raise safety concerns under the intended conditions of use