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1.10.3.2: laccase

This is an abbreviated version!
For detailed information about laccase, go to the full flat file.

Word Map on EC 1.10.3.2

Reaction

4 benzenediol +

O2
= 4 benzosemiquinone + 2 H2O

Synonyms

ATM, benzenediol oxygen oxidoreductase, benzenediol-oxygen oxidoreductase, benzenediol: oxygen oxidoreductase, benzenediol:O2 oxidoreductase, Benzenediol:oxygen oxidoreductase, blue laccase, blue multicopper oxidase, CcLCC6, CotA, CotA laccase, CotA-laccase, CotA-type laccase, CueO, DA2_0547, DcLac1, DcLac2, Diphenol oxidase, EpoA, Ery4, Ery4 laccase, FpLcc1, FpLcc2, GMET_RS10855, Hvo_B0205, LAC, Lac I, Lac II, Lac-3.5, Lac-4.8, LAC1, Lac2, Lac2a, LAC3, Lac4, LacA, Lacc, laccase, laccase 1, laccase 3, laccase A, Laccase allele OR, Laccase allele TS, laccase CueO, laccase POXA3b, laccase-2, laccase2, LacCh, lacTT, LacTv, LacZ1, Lcc, lcc1, Lcc2, Lcc3, Lcc4, Lcc9, LccA, lccdelta, lccgamma, Ligninolytic phenoloxidase, MAL, McoP, MmPPO laccase, MmPPOA, More, MSK laccase, multicopper oxidase, p-benzenediol:dioxygen oxidoreductase, p-diphenol dioxygen oxidoreductase, p-diphenol oxidase, p-diphenol: dioxygen oxidoreductase, p-diphenol:dioxygenoxidoreductase, p-diphenol:O2 oxidoreductase, p-diphenol:oxygen oxidoreductase, p-diphenol:oxygen-oxidoreductase, PCL, phenol oxidase, PM1 laccase, polyphenol oxidase A, POXA1b, POXA1w, POXA2, POXA3 laccase, POXA3a, POXA3b, POXC, PpoA, PsLac1, PsLac2, rlac1338, SLAC, SN4LAC, spore coat A protein, spore coat protein A, SRL1, SvLAC13, SvLAC15, SvLAC50, SvLAC52, SvLAC9, TaLac1, ThL, TTC1370, TthLAC, two-domain laccase, urishiol oxidase, urushiol oxidase, Wlac, YacK, yellow laccase

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.2 laccase

Purification

Purification on EC 1.10.3.2 - laccase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
250fold to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
360fold to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
400fold to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
54fold purification
90.57fold purification with a yield of 11.95%
-
ammonium sulfate precipitation and DEAE column chromatography
Loweporus lividus
-
ammonium sulfate precipitation and Sepharose Q column chromatography
ammonium sulfate precipitation, Q-Sepharose column chromatography and Sephadex G-100 gel filtration
-
ammonium sulfate precipitation, Q-Sepharose column chromatography, HiTrap Q column chromatography, Mono Q column chromatography, and Superdex-200 gel filtration
-
attempts of heterologous expression of the wild-type laccase using a Pichia pastoris secretory expression system are unsuccessful most likely because the enzyme is too unstable and degrades immediately after production. Therefore the stability of the laccase is improved by using a phylogeny-based design method. A mutant laccase is created in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence. The resulting mutant protein is successfully produced using the Pichia pastoris secretory expression system and then purified
both POXA3a and POXA3b
-
combination of anion exchange chromatography, gel filtration and gel slicing
-
DEAE-cellulose column chromatography, CM-cellulose column chromatography, Q-Sepharose column chromatography, and Superdex 75 gel filtration
-
DEAE-cellulose column chromatography, ConA-Sepharose column chromatography, and Toyopearl HW-55 column chromatography
-
expression in Escherichia coli
extracellular recombinant enzyme expressed in Streptomyces lividans to homogeneity
-
further purification of the commercial preparation by anion exchange chromatography
HiTrap DEAE column chromatography and Sephacryl S-200 gel filtration
-
isoform Lcc2
-
isozyme laccase
isozyme SRL1
-
isozymes Lac I, Lac II
Phlebia fascicularia
-
laccase I-II
-
method development for one-step affinity chromatographic purifcation, 46fold purification on 1,4-butanediol diglycidyl ether affinity resin, analysis of affinity chromatographic matrices containing either urea, acetamide, ethanolamine, or iminodiacetic acid, and best with 1,4-butanediol diglycidyl ether, as affinity ligands for enzyme purification from fermentation broth
-
microfiltration, ultrafiltration and acetone precipitation
-
native enzyme 10.3fold by ammonium sulfate fractionation, anion exchange chromatography and gel filtration
-
native enzyme 117fold by hydrophobic interaction chromatography and gel filtration
native enzyme 12.1fold to homogeneity by anion exchange chromatography and gel filtration
-
native enzyme 151.7fold by ultrafiltration, gel filtration, and concanavalin A affinity chromatography
-
native enzyme 16.7fold from strain 577.79 by ultrafiltration, anion exchange chromatography, and gel filtration to homogeneity
-
native enzyme 16fold from fresh fruitig bodies by affinity chromatography, ion exchange chromatography, and gel filtration
-
native enzyme 180fold by cold precipitation, ultrafiltration ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
native enzyme 2.14fold by cation exchange chromatography and gel filtration
-
native enzyme 209fold from strain 951022 by ethanol precipitation, anion exchange and hydrophobic interaction chromatography, and gel filtration, to homogeneity
-
native enzyme 24.3fold by two different steps of anion exchange chromatography, and gel filtration
-
native enzyme 24.9fold from strain WR1, by two steps of anion exchange chromatography to over 96% homogeneity
native enzyme 26fold by ultrafiltration/diafiltration, gel filtration and anion exchange chromatography
-
native enzyme 29fold by a two-step procedure involving heat treatment and immobilized metal affinity chromatography
-
native enzyme 3.61fold by ultrafiltration, anion exchange chromatography, and gel filtration
-
native enzyme 3fold by ammonium sulfate fractionation and anion exchange chromatography
-
native enzyme 45fold from solid-state fermentation on peel from Citrus reticulata to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, ultrafiltration, and gel filtration
-
native enzyme 8.4fold by ultrafiltration, two steps of anion and one of cation exchange chromatography
-
native enzyme 82fold from the culture filtrate after 7 days of growth of the mycelium, by ultrafiltration, anion exchange chromatography, and gel filtration
-
native enzyme 8fold by two teps of anion exchange chromatography, and gel filtration to homogeneity
FJ560721
native enzyme 95.7fold to homogeneity by anion exchange chromatography and gel filtration
native enzyme about 35fold from fruiting bodies by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, and gel filtration
-
native enzyme by ammonium sulfate fractionation, cation exchange chromatography, ultrafiltration, and gel filtration to homogeneity
native enzyme from cladodes to homogeneity by anion exchange chromatography and gel filtration
native enzyme from culture filtrate by anion exchange and hydrophobic interaction chromatography
native enzyme from the pharate pupal cuticle to homogeneity
native enzyme purified 54.1fold by two steps of hydrophobic interaction chromatography
-
native enzyme to homogeneity
native extracelluar enzyme 56fold by acetone precipitation and anion exchange chromatography
-
native extracellular enzyme 16fold from cell-free supernatant by ammonium sulfate fractionation, PEG 20000 concentration, anion exchange chromatography, and gel filtration
-
native extracellular enzyme 250fold to homogeneity by anion exchange chromatographies and gel filtration
-
native extracellular enzyme 3.67fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration to homogeneity
-
native extracellular enzyme 6.6fold from culture supernatant by anion exchange chromatography
-
native extracellular enzyme by a three-stage chromatographic purification to homogeneity
-
native extracellular enzyme by anion exchange chromatography, ammonium sulfate fractionation, and gel filtration
-
native extracellular laccase 10fold by ultrafilatration, anion exchange chromatography, and again ultrafiltration, to homogeneity
-
native isozymes about 50fold by anion exchange chromatography and gel filtration
-
native isozymes Lac I and II by anion exchange chromatography and gel filtration, overview
Fomes sclerodermeus
-
Ni-chelating affinity chromatography
-
one-step purification
-
partial
partial purification by ammonium sulfate precipitation and DEAE-cellulose column chromatography
-
purified by passage on Sepharose derivatized with chloroethylamine followed by chromatography on Phenyl-Sepharose
purified enzyme has a distinct blue color
-
purified to homogeneity by consecutive steps of hydrophobic interaction, anion exchange and size exclusion chromatography
recombinant CotA-type laccase from Escherichia coli strain JM109 by anion exchange chromatography, ultrafiltration, and gel filtration
-
recombinant enzyme
recombinant enzyme 3.8fold by ulrafiltration, anion exchange chromatography, again ultrafiltration, and gel filtration
recombinant enzyme 82fold from Escherichia coli
recombinant enzyme from a Trichoderma reeseicbh1 cbh1 disruption mutant strain
-
recombinant enzyme from Aspergillus oryzae by gel filtration, anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, and ultrafiltration
-
recombinant enzyme from Nicotiana tabacum BY-2 cell culture supernatant by hydrophobic interaction and anion exchange chromatography
recombinant enzyme from Pichia pastoris strain GS115 5fold by hydrophobic interaction chromatography
-
recombinant enzyme Lac1 from Pichia pastoris by ultrafiltration, anion exchange chromatography, ultrafiltration, and gel filtration
recombinant His-taagged enzyme from supercompetent Escherichia coli cells, procedure optimization including affinity chromatography and gel filtration
-
recombinant His-tagged two-type domain laccase from Escherichia coli strain BL21(DE3) by heat treatment, centrifugation, nickel affinity chromatography, and ultrafiltration
-
recombinant His6-tagged wild-type and truncated mutant enzymes from Escherichia coli strain Rosetta 2(DE3) and BL21(DE3) by nickel affinity chromatography, dialysis, ultrafiltration, gel filtration, and again dialysis. The wild-type enzyme is purified by 16fold, the mutant enzyme by 3fold
recombinant protein, by Ni-NTA affinity chromatography
recombinant wild-type and mutant enzymes from Saccharomyces cerevisiae by anion exchange chromatography and gel filtration
recombinant wild-type and mutant enzymes from Saccharomyces cerevisiae by tangential ultrafiltration, dialysis, pressure ultrafiltration, anion exchange chromatography, and gel filtration
recombinant wild-type and mutant enzymes from Trichoderma reesei and Saccharomyces cerevisiae by two different steps of anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration
recombinant wild-type and mutant enzymes partially from Saccharomyces cerevisiae strain W303-1A
the protein is primarily found in inclusion bodies. The resulting insoluble proteins are solubilized with 6 M guanidine HCl and refolded using an on-column refolding procedure
-
three isoenzymes
-
two laccases with distinct pI values, Lac-3.5 and Lac-4.8, are purified from peptone-spruce sawdust-charcoal cultures
-
two step procedure: heat treatment (at 70°C) and Con-A affinity chromatography
-
wild-type and mutant enzymes