1.10.3.2: laccase

This is an abbreviated version!
For detailed information about laccase, go to the full flat file.

Word Map on EC 1.10.3.2

1.10.3.2

trametes

decolor

versicolor

abts

white-rot

manganese

basidiomycete

pleurotus

oxidases

wastewater

textile

effluent

ostreatus

bioremediation

electrode

lignocellulosic

recalcitrant

pulp

guaiacol

straw

wood

tyrosinase

mushroom

solid-state

submerged

cellulase

xylanase

brilliant

phanerochaete

chemoradiation

chrysosporium

malachite

trinuclear

veratryl

kraft

chemoradiotherapy

copper-containing

reusability

monophenols

indigo

edodes

humic

bagasse

brachytherapy

bioelectrocatalytic

copper-binding

azoreductase

monolignols

industry

para-aortic

food industry

degradation

biofuel production

energy production

medicine

environmental protection

biotechnology

analysis

cmcase

synthesis

Reaction

4 benzenediol +

= 4 benzosemiquinone + 2 H2O

Synonyms

ATM, benzenediol oxygen oxidoreductase, benzenediol-oxygen oxidoreductase, benzenediol: oxygen oxidoreductase, benzenediol:O2 oxidoreductase, Benzenediol:oxygen oxidoreductase, blue laccase, blue multicopper oxidase, CcLCC6, CotA, CotA laccase, CotA-laccase, CotA-type laccase, CueO, DA2_0547, DcLac1, DcLac2, Diphenol oxidase, EpoA, Ery4, Ery4 laccase, FpLcc1, FpLcc2, GMET_RS10855, Hvo_B0205, LAC, Lac I, Lac II, Lac-3.5, Lac-4.8, LAC1, Lac2, Lac2a, LAC3, Lac4, LacA, Lacc, laccase, laccase 1, laccase 3, laccase A, Laccase allele OR, Laccase allele TS, laccase CueO, laccase POXA3b, laccase-2, laccase2, LacCh, lacTT, LacTv, LacZ1, Lcc, lcc1, Lcc2, Lcc3, Lcc4, Lcc9, LccA, lccdelta, lccgamma, Ligninolytic phenoloxidase, MAL, McoP, MmPPO laccase, MmPPOA, More, MSK laccase, multicopper oxidase, p-benzenediol:dioxygen oxidoreductase, p-diphenol dioxygen oxidoreductase, p-diphenol oxidase, p-diphenol: dioxygen oxidoreductase, p-diphenol:dioxygenoxidoreductase, p-diphenol:O2 oxidoreductase, p-diphenol:oxygen oxidoreductase, p-diphenol:oxygen-oxidoreductase, PCL, phenol oxidase, PM1 laccase, polyphenol oxidase A, POXA1b, POXA1w, POXA2, POXA3 laccase, POXA3a, POXA3b, POXC, PpoA, PsLac1, PsLac2, rlac1338, SLAC, SN4LAC, spore coat A protein, spore coat protein A, SRL1, SvLAC13, SvLAC15, SvLAC50, SvLAC52, SvLAC9, TaLac1, ThL, TTC1370, TthLAC, two-domain laccase, urishiol oxidase, urushiol oxidase, Wlac, YacK, yellow laccase

ECTree

1 Oxidoreductases

1.10 Acting on diphenols and related substances as donors

1.10.3 With oxygen as acceptor

1.10.3.2 laccase

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Results	in table
42	Activating Compound
8855	AA Sequence
203	Application
1	CAS Registry Number
106	Cloned(Commentary)
19	Crystallization (Commentary)
9	Expression
48	General Information
17	General Stability
13	IC50 Value
803	Inhibitors
257	kcat/KM [mM/s]
15	Ki Value [mM]
741	KM Value [mM]
108	Localization
333	Metals/Ions
243	Molecular Weight [Da]
40	Natural Substrates/ Products (Substrates)
124	Organic Solvent Stability
616	Organism
4	Pathway
349	PDB ID
358	pH Optimum
93	pH Range
164	pH Stability
67	pI Value
81	Posttranslational Modification
162	Protein Variants
204	Purification (Commentary)
9	Reaction
3	Reaction Type
583	Reference
8	Renatured (Commentary)
176	Source Tissue
196	Specific Activity [micromol/min/mg]
19	Storage Stability
1635	Substrates and Products (Substrate)
249	Subunits
318	Synonyms
1	Systematic Name
221	Temperature Optimum [°C]
75	Temperature Range [°C]
317	Temperature Stability [°C]
505	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 1.10.3.2 - laccase

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10000

Trametes versicolor

Q12718, Q5IR80

gel filtration

742173

100000

Melanocarpus albomyces

Q70KY3

x * 70000, recombinant nonglycosylated enzyme expressed in Trichodrma reesei, SDS-PAGE, x * 100000, recombinant glycosylated enzyme expressed in Saccharomyces cerevisiae, SDS-PAGE

697913

107000

Shigella dysenteriae

recombinant mutant WlacD

110000

114000

120000

Toxicodendron vernicifluum

130000

14000

gel filtration

150000 - 151200

about, gel filtration and mass spectrometry

154000

gel filtration

165000

175000

177000

enzyme conjugated with 2 kDa PEG, gel filtration

672747

180000

Bacillus sp. (in: Bacteria)

SDS-PAGE

743116

220000

Leucaena leucocephala

gel filtration

743704

22400

Pleurotus pulmonarius

2 * 22400, SDS-PAGE

260000

gel filtration

269000

enzyme conjugated with 5 kDa PEG, gel filtration

29000

gel filtration

29800

calculated from sequence

765710

31000

Trametes polyzona

764540

32000

Bacillus sp. MSK-01

34000

35500 - 38000

36000

37000

2 * 37000, SDS-PAGE

38000

3 * 38000, SDS-PAGE after boiling for 20 min, 114000, stable migration in regular SDS-PAGE

383000 - 390000

40000

x * 40000, SDS-PAGE

43000

Kitasatospora aureofaciens

2 * 43000, SDS-PAGE

43700

45000

46000

47000

Perenniporia fraxinea

1 * 47000, SDS-PAGE

688328

47600

Perenniporia fraxinea

gel filtration

688328

48000

Bacillus licheniformis

SDS-PAGE

765122

48986

Thermus thermophilus

Q72HW2

calculated from sequence

747726

52000

2 entries

52000 - 59000

Rigidoporus microporus

gel filtration

396353

52700

Geobacter metallireducens

gel filtration

53000

54000

54100

x + 63700, glycosylated recombinant enzyme, SDS-PAGE, x * 54100, about, sequence calculation

55000

56800

x * 56800, SDS-PAGE

57000

57500

x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE

58000

58500

58600

59000

60000

60200

MALDI-TOF

61000

61400

x * 61400, SDS-PAGE

62000

62700

x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE

63000

63200

63397

x * 63397, calculation from sequence

726713

63400

Polyporus brumalis

A3F8Z8

estimated from SDS-PAGE

64000

64000 - 65000

64100

x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE

726424

64200

Podospora anserina

x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE

64800

65000

65000 - 68000

gel filtration, 3 isoenzymes

66000

67000

68000

68500

1 * 68500, SDS-PAGE

69000

69100

gel filtration

69500

70000

70000 - 75000

Kitasatospora aureofaciens

gel filtration

701029

70780

Shiraia sp. SUPER-H168

x * 70780, mass spectrometry, x * 69000, SDS-PAGE

726416

71000

4 entries

71450

Trametes sp.

1 * 74000, SDS-PAGE, 1 * 71450, mass spectrometry

656847

72000

3 entries

72400

Botrytis cinerea

x * 72400, SDS-PAGE, probably anomalous migration on SDS-gels

396352

72700

Myrioconium sp.

matrix-assisted laser desorption/ionization-time of flightmass spectrometry

684626

73000

3 entries

73500

Prunus persica

ultracentrifugation, amino acid composition

74000

75000

76000

77000

79000

79600

x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE

726434

80000

4 entries

82000

2 entries

84000

Didymosphaeria variabile

SDS-PAGE

711502

84280

Bombyx mori

2 * 84280, mature enzyme, calculated from amino acid sequence

712159

85000

4 entries

88000 - 90000

Streptomyces viridochromogenes

gel filtration

90000

92000

96000

1 * 96000, SDS-PAGE

96000 - 110000

97000

additional information

2 entries