1.1.99.35: soluble quinoprotein glucose dehydrogenase
This is an abbreviated version!
For detailed information about soluble quinoprotein glucose dehydrogenase, go to the full flat file.
Word Map on EC 1.1.99.35
-
1.1.99.35
-
calcoaceticus
-
acinetobacter
-
aldose
-
pqqh2
-
electrodes
-
pqq-containing
-
tautomerization
-
hydride
-
pqq-dependent
-
synthesis
-
stopped-flow
-
beta-anomer
-
biofuel production
-
half-reaction
-
gluconolactone
-
disaccharide
-
electrochemical
-
beta-strands
-
energy production
-
diagnostics
-
biotechnology
- 1.1.99.35
- calcoaceticus
-
acinetobacter
- aldose
- pqqh2
-
electrodes
-
pqq-containing
-
tautomerization
-
hydride
-
pqq-dependent
- synthesis
-
stopped-flow
-
beta-anomer
- biofuel production
-
half-reaction
- gluconolactone
- disaccharide
-
electrochemical
-
beta-strands
- energy production
- diagnostics
- biotechnology
Reaction
Synonyms
2,7,9-tricarboxypyrroloquinoline quinone-dependent glucose dehydrogenase, GCD, GDH, GDHB, mGDH, PQQ dependent soluble glucose dehydrogenase, PQQ glucose dehydrogenase, PQQ-dependent glucose dehydrogenase, PQQ-GDH, PQQ-glucose dehydrogenase, PQQ-sGDH, PQQGDH, PQQGDH-B, pyrroloquinoline quinone soluble glucose dehydrogenase, pyrroloquinoline quinone-dependent glucose dehydrogenase, pyrroloquinolinequinone-dependent glucose dehydrogenase, s-GDH, sGDH, sGDH-PQQ, soluble PQQ-dependent glucose dehydrogenase, soluble PQQ-GDH, soluble PQQ-glucose dehydrogenase, soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
ECTree
1.1.99.35 soluble quinoprotein glucose dehydrogenaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 1.1.99.35 - soluble quinoprotein glucose dehydrogenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol
2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
cellobiose + N-methylphenazonium methylsulfate
cellobiono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
-
-
-
?
cellobiose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-allose + N-methylphenazonium methylsulfate
D-allono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
-
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
D-fucose + oxidized 2,6-dichlorophenolindophenol
D-fucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-galactose + N-methylphenazonium methylsulfate
D-galactono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
30% activity compared to D-glucose
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
D-galactose + oxidized N-methylphenazonium methyl sulfate
D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
D-glucose + dimethyl((6-methyl-7-((4-nitrosophenyl)amino)-1H-imidazo[1,2-b]pyrazol-1-yl)methyl)phosphine oxide
D-glucono-1,5-lactone + reduced dimethyl((6-methyl-7-((4-nitrosophenyl)amino)-1H-imidazo[1,2-b]pyrazol-1-yl)methyl)phosphine oxide
-
-
-
?
D-glucose + N-methylphenazonium methylsulfate
D-glucono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
100% activity
-
-
?
D-glucose + osmium polymer
D-glucono-1,5-lactone + reduced osmium polymer
-
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenol-indophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
D-glucose + oxidized N-methylphenazonium methyl sulfate
D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
-
?
D-lyxose + N-methylphenazonium methylsulfate
D-lyxono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
-
-
-
?
D-lyxose + oxidized 2,6-dichlorophenolindophenol
D-lyxono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-mannose + N-methylphenazonium methylsulfate
D-mannono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
-
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
D-ribose + N-methylphenazonium methylsulfate
D-ribono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
5% activity compared to D-glucose
-
-
?
D-ribose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-xylose + N-methylphenazonium methylsulfate
D-xylono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
14% activity compared to D-glucose
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
D-xylose + oxidized N-methylphenazonium methyl sulfate
D-xylono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
-
?
L-arabinose + N-methylphenazonium methylsulfate
L-arabinono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
-
-
-
?
L-arabinose + oxidized 2,6-dichlorophenolindophenol
L-arabinono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
lactose + 1-(N,N-dimethylamine)-4-(4-morpholine)benzene
lactono-1,5-lactone + reduced 1-(N,N-dimethylamine)-4-(4-morpholine)benzene
-
-
-
?
lactose + N,N'-dimethyl-4,4'-azopyridinium methyl sulfate
lactono-1,5-lactone + reduced N,N'-dimethyl-4,4'-azopyridinium methyl sulfate
-
-
-
?
lactose + N-methylphenazonium methylsulfate
lactono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
4% activity compared to D-glucose
-
-
?
lactose + oxidized 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
lactose + oxidized N-methylphenazonium methyl sulfate
4-O-beta-D-galactopyranosyl-D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
-
-
-
?
maltose + acceptor
maltono-1,5-lactone + reduced acceptor
-
-
-
?
maltose + N-methylphenazonium methylsulfate
maltono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
12% activity compared to D-glucose
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
melibiose + N-methylphenazonium methylsulfate
melibiono-1,5-lactone + reduced N-methylphenazonium methylsulfate
-
5% activity compared to D-glucose
-
-
?
melibiose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol
2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol
2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
4% of the activity with D-glucose
-
-
?
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
81% of the activity with D-glucose
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
47% of the activity with D-glucose
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
11% of the activity with D-glucose
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
about 13% activity compared to D-glucose
-
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
about 13% activity compared to D-glucose
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
-
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
100% activity
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
100% activity
-
-
?
D-glucose + phenazine methosulfate
D-glucono-1,5-lactone + reduced phenazine methosulfate
-
-
-
-
?
D-glucose + phenazine methosulfate
D-glucono-1,5-lactone + reduced phenazine methosulfate
-
-
-
-
?
D-glucose + phenazine methosulfate
D-glucono-1,5-lactone + reduced phenazine methosulfate
-
-
-
-
?
D-glucose + phenazine methosulfate
D-glucono-1,5-lactone + reduced phenazine methosulfate
-
-
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
13% of the activity with D-glucose
-
-
?
D-mannose + oxidized 2,6-dichlorophenolindophenol
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
about 11% activity compared to D-glucose
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
7% of the activity with D-glucose
-
-
?
D-xylose + oxidized 2,6-dichlorophenolindophenol
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
about 9% activity compared to D-glucose
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
61% of the activity with D-glucose
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
61% of the activity with D-glucose
-
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
about 50% activity compared to D-glucose
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
61% of the activity with D-glucose
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
61% of the activity with D-glucose
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
about 70% activity compared to D-glucose
-
-
?
additional information
?
-
-
kinetic mechanism of sGDH consists of (a) step(s) in which a fluorescing intermediate is formed, and a subsequent, irreversible step, determining the overall rate of the reductive half-reaction. The pH optimum determined for glucose oxidation appears to be 7.0, implying that reoxidation of sGDHred is rate-limiting with those electron acceptors displaying a different value under steady-state conditions.
-
-
?
additional information
?
-
-
mechanism comprises general base-catalyzed hydride transfer
-
-
?
additional information
?
-
-
the sequential steps in the mechanism of sGDH must be reversible substrate binding, direct transfer of a hydride ion (reversible or irreversible) from the C1 position of the beta-anomer of glucose to the C5 of PQQ, irreversible, rate-determining tautomerization of the fluorescing, C5-reduced PQQ to PQQH2 and release (or earlier) of the product, D-glucono-delta-lactone, and oxidation of PQQH2 by an electron acceptor. The PQQ-activating Ca2+ greatly facilitates the reactions occurring in the second step. His144 may also play a role in this by acting as a general base catalyst, initiating hydride transfer by abstracting a proton from the anomeric OH group of glucose
-
-
?
additional information
?
-
-
almost no activity with D-fructose
-
-
?
additional information
?
-
-
no activity with D-glucosamine, L-altrose, L-lyxose, L-talose, L-mannose, D-altrose, D-arabinose, D-talose, L-xylose, L-glucose, L-galactose, L-allose, sucrose, trehalose, and raffinose
-
-
?
additional information
?
-
-
almost no activity with D-fructose
-
-
?
