1.1.99.21: D-sorbitol dehydrogenase (acceptor)

This is an abbreviated version!
For detailed information about D-sorbitol dehydrogenase (acceptor), go to the full flat file.

Word Map on EC 1.1.99.21

Reaction

D-sorbitol
+
acceptor
=
L-sorbose
+
reduced acceptor

Synonyms

D-sorbitol dehydrogenase, FAD-SLDH, GoSLDH, SDH, SLDH, sldhAB, sorbitol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.99 With unknown physiological acceptors
                1.1.99.21 D-sorbitol dehydrogenase (acceptor)

Purification

Purification on EC 1.1.99.21 - D-sorbitol dehydrogenase (acceptor)

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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
by cell disruption and ultracentrifugation
-
by CM-cellulose and sephacryl HR 400 column chromatography
-
ion exchange chromatography, gel filtration
-
metal affinity column chromatography
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)-CodonPlus RIL
-