1.1.99.21: D-sorbitol dehydrogenase (acceptor)

This is an abbreviated version, for detailed information about D-sorbitol dehydrogenase (acceptor), go to the full flat file.

Reaction

D-sorbitol
+
acceptor
=
L-sorbose
+
reduced acceptor

Synonyms

D-sorbitol dehydrogenase, FAD-SLDH, GoSLDH, SDH, SLDH, sldhAB, sorbitol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.99 With unknown physiological acceptors
                1.1.99.21 D-sorbitol dehydrogenase (acceptor)

Cloned

Cloned on EC 1.1.99.21 - D-sorbitol dehydrogenase (acceptor)

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21-Gold (DE3) cells
-
gene sldh, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)-CodonPlus RIL
-
gene sldhAB, enzyme overexpression in an industrial strain Gluconobacter oxydans WSH-003 with a strong promoter PtufB, subcloning in Escherichia coli strain JM109, quantitative real-time PCR expression analysis
in Escherichia coli, plasmids harboring the disrupted gene are introduced into Gluconobacter frateurii THD32 by electroporation
-