1.1.99.14: glycolate dehydrogenase

This is an abbreviated version!
For detailed information about glycolate dehydrogenase, go to the full flat file.

Reaction

Synonyms

GADH, GDH, GlcD, GlcD1, GlcD2, GlcDH, glycolate dehydrogenase, glycolate dehydrogenase I, glycolate oxidoreductase, glycolic acid dehydrogenase, More

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.99 With unknown physiological acceptors

                1.1.99.14 glycolate dehydrogenase

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Results	in table
1	Activating Compound
16730	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
10	Cofactor
1	General Information
38	Inhibitors
2	kcat/KM [mM/s]
2	Ki Value [mM]
12	KM Value [mM]
9	Localization
5	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
42	Organism
10	Pathway
5	pH Optimum
1	pH Range
1	pH Stability
1	pI Value
1	Protein Variants
3	Purification (Commentary)
1	Reaction
3	Reaction Type
20	Reference
1	Source Tissue
11	Specific Activity [micromol/min/mg]
1	Storage Stability
46	Substrates and Products (Substrate)
2	Subunits
18	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Stability [°C]
3	Turnover Number [1/s]

Purification

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Purification on EC 1.1.99.14 - glycolate dehydrogenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

ammonium sulfate precipitation, DEAE-Toyopearl column chromatography, Q-Toyopearl column chromatography, and Toyopearl, GigaCap Q-Toyopearl column chromatography, hydroxyapatite column chromatography, and gel filtration

Trichoderma harzianum

-

725738

partially, from cells grown on glycolate

Escherichia coli

-

348285

recombinant N-terminally Strep-tagged enzyme from Escherichia coli strain BL21 by streptavidin-tactin affinity chromatography

Synechocystis sp.

-

676615