1.1.98.7: Ser-type anaerobic sulfatase-maturating enzyme
This is an abbreviated version!
For detailed information about Ser-type anaerobic sulfatase-maturating enzyme, go to the full flat file.
Reaction
Synonyms
anaerobic sulfatase-maturating enzyme, anSME, AtsB, cpe0635, nSMEcpe, Ser-type anaerobic sulfatase-maturating enzyme
ECTree
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Substrates Products
Substrates Products on EC 1.1.98.7 - Ser-type anaerobic sulfatase-maturating enzyme
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REACTION DIAGRAM
Ac-AVPSSIPSRASILTGM-NH2 + S-adenosyl-L-methionine + dithionite
Ac-AVPS-3-oxo-L-Ala-IPSRASILTGM-NH2 + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O
Ac-FENAYTAVPSSIASRASILTGMS-NH2 + S-adenosyl-L-methionine
Ac-FENAYTAVPS-3-oxo-L-Ala-IASRASILTGMS-NH2 + L-methionine + 5'-deoxyadenosine
Ac-YYTSPMSAPARSMLLTGN + S-adenosyl-L-methionine
Ac-YYTSPM-3-oxo-L-Ala-APARSMLLTGN + L-methionine + 5'-deoxyadenosine
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite
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Ac-YYTSPMTAPARSMLLTGN + S-adenosyl-L-methionine
Ac-YYTSPM-(2S)-2-amino-3-oxobutanoyl-APARSMLLTGN + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite
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acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine
acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-3-oxo-L-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine
S-adenosyl-L-methionine + a [Klebsiella pneumoniae sulfatase]-L-serine
[Klebsiella pneumoniae sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
the atsB gene product plays a posttranslational role that is essential for the sulfatase to gain its catalytic activity
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S-adenosyl-L-methionine + a [sulfatase]-L-serine
a [sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
S-adenosyl-L-methionine + a [sulfatase]-L-serine
[sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
AtsB from Klebsiella pneumoniae can act on sulfatases of other species. The cytosolic cysteine-type sulfatase of Pseudomonas aeruginosa can be converted into a substrate of Klebsiella pneumoniae AtsB if the cysteine is substituted by serine and a signal peptide is added
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S-adenosyl-L-methionine + [Klebsiella pneumoniae sulfatase]-L-serine
[Klebsiella pneumoniae sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
peptide analysis of the Klebsiella pneumoniae sulfatase protein expressed in the presence of AtsB reveales that half of the polypeptides carry the formylglycine at position 72, while the remaining polypeptides carry the encoded serine. The inactive sulfatase expressed in the absence of AtsB carries exclusively serine 72, demonstrating that the atsB gene is required for formylglycine modification
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S-adenosyl-L-methionine + [periplasmic sulfatase AtsA]-L-serine
[periplasmic sulfatase AtsA]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine + dithionite
Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-3-oxo-L-Ala-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O
[arylsulfatase]-L-serine + S-adenosyl-L-methionine + reduced acceptor
[arylsulfatase]-3-oxo-L-alanine + L-methionine + 5'-deoxyadenosine + acceptor + H2O
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Ac-AVPS-3-oxo-L-Ala-IPSRASILTGM-NH2 + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite
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?
Ac-AVPSSIPSRASILTGM-NH2 + S-adenosyl-L-methionine + dithionite
Ac-AVPS-3-oxo-L-Ala-IPSRASILTGM-NH2 + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite
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?
Ac-FENAYTAVPS-3-oxo-L-Ala-IASRASILTGMS-NH2 + L-methionine + 5'-deoxyadenosine
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Ac-FENAYTAVPSSIASRASILTGMS-NH2 + S-adenosyl-L-methionine
Ac-FENAYTAVPS-3-oxo-L-Ala-IASRASILTGMS-NH2 + L-methionine + 5'-deoxyadenosine
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acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine
acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-3-oxo-L-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite with higly reduced activity
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acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine
acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-3-oxo-L-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite with higly reduced activity
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a [sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
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S-adenosyl-L-methionine + a [sulfatase]-L-serine
a [sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
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S-adenosyl-L-methionine + a [sulfatase]-L-serine
a [sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
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the enzyme converts nonclassical aldehyde tags of the SX(A/P)XR-type. The enzyme is able to modify the artificial serine-containing motif STAGR
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[periplasmic sulfatase AtsA]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
the enzyme is strictly required for modification of Ser72 in the periplasmic sulfatase AtsA of Klebsiella pneumoniae. AtsB-mediated formation of 3-oxo-L-alanine is dependent on the signal peptide of AtsA
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S-adenosyl-L-methionine + [periplasmic sulfatase AtsA]-L-serine
[periplasmic sulfatase AtsA]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
AtsB-mediated formation of 3-oxo-L-alanine is dependent on the signal peptide of AtsA. AtsB physically interacts with AtsA in a Ser72-dependent manner
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Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine + dithionite
Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-3-oxo-L-Ala-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite with higly reduced activity
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Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine + dithionite
Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-3-oxo-L-Ala-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite with higly reduced activity
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the enzyme converts a serine residue of arylsulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases
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additional information
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the enzyme converts a serine residue of arylsulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases
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additional information
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the enzme also perfoms the reaction of the Cys-type anaerobic sulfatase-maturating enzyme, converting a cysteine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases
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additional information
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the enzme also perfoms the reaction of the Cys-type anaerobic sulfatase-maturating enzyme, converting a serine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases. No reaction with Ac-YYTSPM(allo)TAPARSMLLTGN
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additional information
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the enzme also perfoms the reaction of the Cys-type anaerobic sulfatase-maturating enzyme, converting a cysteine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases
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additional information
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the enzme also perfoms the reaction of the Cys-type anaerobic sulfatase-maturating enzyme, converting a cysteine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases. No reaction with Tyr-Tyr-Thr-Ser-Pro-Met-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH
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additional information
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the enzyme converts a serine residue of arylsulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases
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additional information
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the enzme also perfoms the reaction of the Cys-type anaerobic sulfatase-maturating enzyme, converting a cysteine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases. No reaction with Tyr-Tyr-Thr-Ser-Pro-Met-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH
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