1.1.98.6: ribonucleoside-triphosphate reductase (formate)

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For detailed information about ribonucleoside-triphosphate reductase (formate), go to the full flat file.

Word Map on EC 1.1.98.6

Reaction

ribonucleoside 5'-triphosphate
+
formate
=
2'-deoxyribonucleoside 5'-triphosphate
+
CO2
+
H2O

Synonyms

anaerobic ribonucleoside-triphosphate reductase, anaerobic ribonucleotide reductase, class III ribonucleoside-triphosphate reductase, nrdD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.98 With other, known, physiological acceptors
                1.1.98.6 ribonucleoside-triphosphate reductase (formate)

Crystallization

Crystallization on EC 1.1.98.6 - ribonucleoside-triphosphate reductase (formate)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
quantum chemical calculation of reaction mechanism. Carbon dioxide is formed from formate, which is present as a cofactor. The formate first forms a formyl radical. The next step, where the formyl radical protonates the 3'-keto group of the substrate, is rate limiting with a calculated total barrier of 19.9 kcal/mol, in reasonable agreement with the experimental rate-limiting barrier of 17 kcal/mol. Zero-point and entropy effects are quite significant in lowering the barrier
-
C-terminal region contains a Zn(Cys)4 center structurally related to the zinc ribbon motif and to rubredoxin and rubrerythrin. Residues Cys543, Cys546, Cys561, and Cys564 coordinate the metal ion tetrahedrally