1.1.5.9: glucose 1-dehydrogenase (FAD, quinone)
This is an abbreviated version!
For detailed information about glucose 1-dehydrogenase (FAD, quinone), go to the full flat file.
Word Map on EC 1.1.5.9
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1.1.5.9
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electrode
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bioanode
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transfer-type
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bioelectrocatalytic
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glomerella
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fungi-derived
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analysis
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cingulata
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biofuel production
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diagnostics
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medicine
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biotechnology
- 1.1.5.9
-
electrode
-
bioanode
-
transfer-type
-
bioelectrocatalytic
- glomerella
-
fungi-derived
- analysis
- cingulata
- biofuel production
- diagnostics
- medicine
- biotechnology
Reaction
Synonyms
D-glucose:acceptor 1-oxidoreductase, dehydrogenase, glucose (acceptor), dehydrogenase, glucose (Aspergillus), EC 1.1.99.10, FAD dependent glucose dehydrogenase, FAD glucose dehydrogenase, FAD-dependent GDH, FAD-dependent glucose dehydrogenase, FAD-GDH, FAD-glucose dehydrogenase, FAD-glucose dehydrogenases, FADGDH, FADGH, flavin adenine dinucleotide dependent glucose dehydrogenase, flavin adenine dinucleotide-dependent glucose dehydrogenase, GDH, GDH-FAD, GLD, glucose dehydrogenase, glucose dehydrogenase (Aspergillus), glucose dehydrogenase (decarboxylating), thermostable GDH, thermostable glucose dehydrogenase
ECTree
1.1.5.9 glucose 1-dehydrogenase (FAD, quinone)Advanced search results
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results (Temperature Stability
Temperature Stability on EC 1.1.5.9 - glucose 1-dehydrogenase (FAD, quinone)
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
25 - 40
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the enzyme remains stable after 15 min at 25-40°C, then activity drops to about 70% at 50°C and is completely lost at 55°C
40 - 60
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after heat treatment at 40-60°C for 15 min, the enzyme maintains its activity close to 100%
40 - 62
unglycosylated enzym e is stable at temperatures up to 45°C , while glycosylatedenzyme maintains 75% activity at 60°C. The melting temperature of the glycosylated enzyme is 62.5°C
40 - 70
after heat treatment at 40-70°C for 15 min, unglycosylated enzyme maintains nearly 100% activity at temperatures up to 45°C, but completely loses activity at 60°C, while glycosylated enzyme displays high thermostability, maintaining 89% activity at 60°C. The melting temperature of the glycosylated enzyme is 66.4°C
45
5 - 70
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enzyme is stable at temperatures between 5-37°C for 30 min, after incubating enzyme at 45°C for 30 min 40% of enzyme activity remains, at 70°C for 30 min 10% of enzyme activity remains
5 - 75
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after preincubation at 70°C for 30 min the enzyme is stable at temperatures between 5-50°C for 30 min, after incubating enzyme at 70°C for 30 min 70% of activity remains
51.6
melting temperature, unglycosylated recombinant protein
56
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pH-dependent thermal stability with the highest Tm values in the acidic range of pH 4.5 to pH 6.4. The maximum Tm value of 56°C is measured in 50 mM sodium acetate buffer pH 5.0 and in 50 mM MES buffer pH 5.8
60
65
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half-life of native enzyme: 2.5 min, half life of enzyme cross-linked to glutaraldehyde: 72 min
45
the wild type enzyme is inactivated rapidly at 45°C, with less than 20% of the initial activity remaining after 25 min and a half-life of inactivation of less than 10 min
60
the enzyme displays very high thermal stability with a half-life of 82 min at 60°C
60
15 min, unglycosylated recombinant protein, complete loss of activity, glycosylated recombinant protein, 89% residual activity
60
15 min, unglycosylated recombinant protein, complete loss of activity, glycosylated recombinant protein, 75% residual activity
