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1.1.5.9: glucose 1-dehydrogenase (FAD, quinone)

This is an abbreviated version!
For detailed information about glucose 1-dehydrogenase (FAD, quinone), go to the full flat file.

Word Map on EC 1.1.5.9

Reaction

D-glucose
+
a quinone
=
D-glucono-1,5-lactone
+
a quinol

Synonyms

D-glucose:acceptor 1-oxidoreductase, dehydrogenase, glucose (acceptor), dehydrogenase, glucose (Aspergillus), EC 1.1.99.10, FAD dependent glucose dehydrogenase, FAD glucose dehydrogenase, FAD-dependent GDH, FAD-dependent glucose dehydrogenase, FAD-GDH, FAD-glucose dehydrogenase, FAD-glucose dehydrogenases, FADGDH, FADGH, flavin adenine dinucleotide dependent glucose dehydrogenase, flavin adenine dinucleotide-dependent glucose dehydrogenase, GDH, GDH-FAD, GLD, glucose dehydrogenase, glucose dehydrogenase (Aspergillus), glucose dehydrogenase (decarboxylating), thermostable GDH, thermostable glucose dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.5 With a quinone or similar compound as acceptor
                1.1.5.9 glucose 1-dehydrogenase (FAD, quinone)

Purification

Purification on EC 1.1.5.9 - glucose 1-dehydrogenase (FAD, quinone)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by DEAE-5PW and Superdex-200 10/300GL column chromatography
-
DEAE-5PW column chromatography and Superdex-200 gel filtration
-
HisTrap nickel affinity chromatography
-
isopropanol precipitation and nickel affinity column chromatography
native extracellular enzyme 23.7fold from culture supernatant by anion exchange and hydrophobic interaction chromatography, ammonium sulfate fractionation, and ultrafiltration
-
Ni-NTA agarose column chromatography
recombinant enzyme from Escherichia coli strain BL21RIL
-
recombinant enzyme from Pichia pastoris strain X-33 5.1fold by hydrophobic interaction and anion exchange chromatography
-
recombinant enzyme from Pichia pastoris to homogeneity
-
resource Q column chromatography
Toyopearl Butyl-650C column chromatography, DEAE Cellufine A-500m column chromatography, and Q-Sepharose column chromatography
-
Toyopearl-butyl 650C column chromatography and SP-Sepharose column chromatography
-