1.1.3.B5: eugenol oxidase
This is an abbreviated version!
For detailed information about eugenol oxidase, go to the full flat file.
Reaction
Synonyms
EUGO, RHA1_ro03282
ECTree
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Application
Application on EC 1.1.3.B5 - eugenol oxidase
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analysis
development of a screening assay for the substrate specificity of para-phenol oxidases based on the detection of hydrogen peroxide using the ferric-xylenol orange complex method
synthesis
fusion of eugenol oxidase and 5-hydroxymethylfurfural oxidase to be used for dioxygen-driven, one-pot, two-step cascade reactions to convert vanillyl alcohol into divanillin and eugenol into lignin oligomers
synthesis
synthesis of syringaresinol in a one-pot conversion containing eugenol oxidase (EUGO) and horseradish peroxidase (HRP) using 2,6-dimethoxy-4-allylphenol as a substrate. The hydrogen peroxide generated from the reaction of EUGO with the substrate is utilized by the HRP to convert the formed sinapyl alcohol into syringaresinol. Mutant I427A together with HRP are capable of efficiently producing syringaresinol as a major product. After optimization and upscaling to a semipreparative scale (1 gr), syringaresinol is obtained in 81% yield