1.1.3.48: 3-deoxy-alpha-D-manno-octulosonate 8-oxidase

This is an abbreviated version, for detailed information about 3-deoxy-alpha-D-manno-octulosonate 8-oxidase, go to the full flat file.

Reaction

3-deoxy-alpha-D-manno-octulopyranosonate
+
O2
=
3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate
+
H2O2

Synonyms

kdnB

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.48 3-deoxy-alpha-D-manno-octulosonate 8-oxidase

Cloned

Cloned on EC 1.1.3.48 - 3-deoxy-alpha-D-manno-octulosonate 8-oxidase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta2-(DE3) cells
expression in Escherichia coli; gene kdnB, encoded in the Kdo8N biosynthetic cluster, DNA and amino acid sequence determination and analysis, subcloning and expression in Escherichia coli strains EC100D and WBB06 at 25°C, recombinant expression of the gene cluster for 8-amino-3,8-dideoxy-D-manno-octulosonic acid biosynthesis from Shewanella oneidensis in Escherichia coli results in lipid A containing 8-amino-3,8-dideoxy-D-manno-octulosonic acid, and in vitro assays confirm the enzymatic functionality converting 3-deoxy-D-manno-octulosonic acid to 8-amino-3,8-dideoxy-D-manno-octulosonic acid, with incorporation into the Kdo8N-lipid A domain of LPS by a metal-dependent oxidase followed by a glutamate-dependent aminotransferase, recombinant expression of His-tagged enzyme in Escherichia coi strain C41