1.1.3.46: 4-hydroxymandelate oxidase
This is an abbreviated version!
For detailed information about 4-hydroxymandelate oxidase, go to the full flat file.
Word Map on EC 1.1.3.46
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1.1.3.46
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oxidases
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fmn-dependent
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amycolatopsis
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four-electron
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flavin
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coelicolor
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benzoylformate
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two-electron
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mononucleotide
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vancomycin
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nonribosomal
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orientalis
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chloroeremomycin
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synthesis
- 1.1.3.46
- oxidases
-
fmn-dependent
-
amycolatopsis
-
four-electron
- flavin
- coelicolor
- benzoylformate
-
two-electron
- mononucleotide
- vancomycin
-
nonribosomal
- orientalis
- chloroeremomycin
- synthesis
Reaction
Synonyms
4HmO, fegC, HmO, hydroxymandelate oxidase, L-4-hydroxymandelate oxidase, p-hydroxymandelate oxidase, Sco3228
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Application
Application on EC 1.1.3.46 - 4-hydroxymandelate oxidase
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synthesis
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whole-cell bioconversion of L-tyrosine into protocatechuic acid using artificial enzymatic cascades engineered in Escherichia coli. The first biocatalytic route comprises L-amino acid deaminase (LAAD) from Proteus mirabilis, hydroxymandelate synthase (HmaS) from Amycolatopsis orientalis, two-component flavin-dependent monooxygenase (HpaBC) from Escherichia coli, hydroxymandelate oxidase (HMO) from Streptomyces coelicolor, benzoylformate decarboxylase (BFD) from Pseudomonas putida, and aldehyde dehydrogenase (ALDH) from Saccharomyces cerevisiae. Combining LAAD/HmaS/HpaBC results in efficient synthesis of 3,4-dihydroxymandelate, which can be further converted to protocatechuic acid by HMO/BFD/ALDH to a final conversion of 64.4%. The recombinant E. coli catalyzes >99% conversion of L-tyrosine into 4-hydroxybenzoate within 12 h, further incorporation of 4-hydroxybenzoate hydroxylase PobA results in complete conversion of 4-hydroxybenzoate into protocatechuic acid, reaching >99% conversion