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1.1.3.46: 4-hydroxymandelate oxidase

This is an abbreviated version!
For detailed information about 4-hydroxymandelate oxidase, go to the full flat file.

Word Map on EC 1.1.3.46

Reaction

(S)-4-hydroxymandelate
+
O2
=
2-(4-hydroxyphenyl)-2-oxoacetate
+
H2O2

Synonyms

4HmO, fegC, HmO, hydroxymandelate oxidase, L-4-hydroxymandelate oxidase, p-hydroxymandelate oxidase, Sco3228

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.46 4-hydroxymandelate oxidase

Application

Application on EC 1.1.3.46 - 4-hydroxymandelate oxidase

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
whole-cell bioconversion of L-tyrosine into protocatechuic acid using artificial enzymatic cascades engineered in Escherichia coli. The first biocatalytic route comprises L-amino acid deaminase (LAAD) from Proteus mirabilis, hydroxymandelate synthase (HmaS) from Amycolatopsis orientalis, two-component flavin-dependent monooxygenase (HpaBC) from Escherichia coli, hydroxymandelate oxidase (HMO) from Streptomyces coelicolor, benzoylformate decarboxylase (BFD) from Pseudomonas putida, and aldehyde dehydrogenase (ALDH) from Saccharomyces cerevisiae. Combining LAAD/HmaS/HpaBC results in efficient synthesis of 3,4-dihydroxymandelate, which can be further converted to protocatechuic acid by HMO/BFD/ALDH to a final conversion of 64.4%. The recombinant E. coli catalyzes >99% conversion of L-tyrosine into 4-hydroxybenzoate within 12 h, further incorporation of 4-hydroxybenzoate hydroxylase PobA results in complete conversion of 4-hydroxybenzoate into protocatechuic acid, reaching >99% conversion