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1.1.3.4: glucose oxidase

This is an abbreviated version!
For detailed information about glucose oxidase, go to the full flat file.

Word Map on EC 1.1.3.4

Reaction

beta-D-glucose
+
O2
=
D-glucono-1,5-lactone
+
H2O2

Synonyms

AldO, beta-D-glucose oxidase, beta-D-glucose oxygen-1-oxidoreductase, beta-D-glucose/oxygen 1-oxidoreductase, beta-D-glucose: oxygen 1-oxidoreductase, beta-D-glucose:O2 1-oxidoreductase, beta-D-glucose:O2-1-oxidoreductase, beta-D-glucose:oxygen 1-oxido-reductase, beta-D-glucose:oxygen 1-oxidoreductase, beta-D-glucose:oxygen oxidoreductase, beta-D-glucose:oxygen-1-oxidoreductase, beta-D-glucose:quinone oxidoreductase, CngoxA, corylophyline, D-glucose oxidase, D-glucose-1-oxidase, deoxin-1, glucose aerodehydrogenase, glucose oxyhydrase, glucose-1-oxidase, GO-2, GOD, GOX, GOxP5, microcid, More, notatin, oxidase, glucose, pen-GOx, penatin, yGOXpenag

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.4 glucose oxidase

pH Range

pH Range on EC 1.1.3.4 - glucose oxidase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1 - 7.5
trend of the dependence of pH on peak current investigated in 0.1 M KCl solution, maximum amperometric response of hydrogen peroxide on the microelectrode dependent on pH over a range from 5.0 to 7.0
2 - 7
-
the activities of modified GOD are about 40-55% of those of native GOD in the pH range tested (pH 2.0-7.0)
2.5 - 8.5
-
activity range, profile overview. 85% of maximal activity at pH 5.0, 51% activity at pH 7.0
2.5 - 9
-
immobilized on activated carbon
3 - 8
3 - 9
3.4 - 7.5
-
soluble enzyme
3.5 - 6.5
-
-
3.5 - 9
activity range of wild-type and mutant enzymes, profile overview
4 - 7
4 - 8
more than 88% of the maximum activity is observed between pH 4.0 and 8.0, outside this range the activity decreases dramatically
4.5 - 10
-
pH 4.5: about 85% of maximal activity, natural GOD and pmr1DELTA-mutant-derived GOD, about 90% of maximal activity, hyperglycosylated GOD expressed in wild-type cells of Saccharomyces cerevisiae, pH 10: about 75% of maximal activity, natural GOD and pmr1DELTA-mutant-derived GOD, about 90% of maximal activity, hyperglycosylated GOD expressed in wild-type cells of Saccharomyces cerevisiae
4.5 - 6.5
-
over 60% activity within this range at 50°C
4.5 - 7
-
pH 4.5: about 60% of maximal activity, pH 7.0: about 50% of maximal activity
4.9 - 8.9
-
70% of maximal activity at pH 4.9 and pH 8.9
5 - 10
pH 5.0: about 60% of maximal activity, pH 10.0: about 40% of maximal activity, recombinant wild-type enzyme
5 - 7.5
-
under O2 and Ar at 37°C, recombinant enzyme yGOXpenag
5.5 - 9
-
80% of the maximal activity is observed in the pH 5.5-9.0 range
6 - 9
-
over 80% of maximal activity within this range
additional information
-
the pH correlation of enzyme activity and catalytic characteristics in various buffer systems (phosphate (pH 5.0–9.0), citrate (pH 3.0–5.0), citrate-phosphate (pH 3.0–9.0), and universal (pH 3.0-9.0)) are determined. The GOx is the most efficiently interacting with substrate (glucose) in phosphate buffer at pH 7.0