1.1.3.12: pyridoxine 4-oxidase
This is an abbreviated version!
For detailed information about pyridoxine 4-oxidase, go to the full flat file.
Word Map on EC 1.1.3.12
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1.1.3.12
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pyridoxal
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mesorhizobium
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loti
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fad
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synthesis
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chaperonins
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nitrogen-fixing
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luteolum
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microbacterium
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4-dehydrogenase
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symbiotic
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pyridoxamine
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pyridoxamine-pyruvate
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hydride
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aminotransferase
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glucose-methanol-choline
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fad-dependent
- 1.1.3.12
- pyridoxal
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mesorhizobium
- loti
- fad
- synthesis
- chaperonins
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nitrogen-fixing
- luteolum
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microbacterium
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4-dehydrogenase
-
symbiotic
- pyridoxamine
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pyridoxamine-pyruvate
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hydride
- aminotransferase
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glucose-methanol-choline
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fad-dependent
Reaction
Synonyms
oxidase, pyridoxol 4-, PN 4-oxidase, PNO, PNOX, pyridoxin 4-oxidase, pyridoxine-4-oxidase, pyridoxol 4-oxidase
ECTree
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Application
Application on EC 1.1.3.12 - pyridoxine 4-oxidase
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synthesis
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coexpression of enzyme with GroEL/Es genes in Escherichia coli under cold stress at 23°C results in 88fold higher specific activity than that of Microbacterium luteolum
synthesis
development of a simple and efficient synthesis for 4-pyridoxolactone starting with pyridoxine and using a whole-cell biotransformation by two transformed Escherichia coli cell type expressing the pyridoxine oxidase and the catalase, and chaperonin, while the second set expressed pyridoxal 4-dehydrogenase, overview. Pyridoxine is first oxidized to pyridoxal, which is then dehydrogenated to 4-pyridoxolactone by pyridoxine 4-oxidase and pyridoxal 4-dehydrogenase, respectively
synthesis
bioconversion of pyridoxine to pyridoxal. Approximately 450 mM pyridoxal is produced from 500 mM pyridoxine using recombinant Rhodococcus erythropolis expressing the pyridoxine 4-oxidase gene. Pyridoxal is converted to pyridoxamine by expression of the pyridoxamine-pyruvate aminotransferase gene derived from Rhizobium loti, and pyridoxine to pyridoxamine through pyridoxal using coexpression of the genes for pyridoxine 4-oxidase and pyridoxamine-pyruvate aminotransferase