1.1.3.10: pyranose oxidase

This is an abbreviated version!
For detailed information about pyranose oxidase, go to the full flat file.

Reaction

Synonyms

C-2 specific pyranose-2-oxidase, carbohydrate oxidase, glucose 2-oxidase, glucose-2-oxidase, P2O, P2Ox, POX, PROD, PyOx, pyranose 2-Oxidase, pyranose oxidase, pyranose-2-oxidase, pyranose/oxygen 2-oxidoreductase, pyranose: oxygen 2-oxidoreductase, pyranose:oxygen 2-oxidoreductase, pyranose:oxygen-2-oxidoreductase, TmP2Ox

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.3 With oxygen as acceptor

                1.1.3.10 pyranose oxidase

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Results	in table
3	Activating Compound
183	AA Sequence
20	Application
1	CAS Registry Number
31	Cloned(Commentary)
34	Cofactor
8	Crystallization (Commentary)
7	General Information
2	General Stability
70	Inhibitors
174	kcat/KM [mM/s]
4	Ki Value [mM]
496	KM Value [mM]
6	Localization
10	Metals/Ions
45	Molecular Weight [Da]
32	Natural Substrates/ Products (Substrates)
110	Organism
149	PDB ID
37	pH Optimum
9	pH Range
26	pH Stability
2	pI Value
3	Posttranslational Modification
115	Protein Variants
51	Purification (Commentary)
8	Reaction
4	Reaction Type
107	Reference
13	Source Tissue
58	Specific Activity [micromol/min/mg]
16	Storage Stability
400	Substrates and Products (Substrate)
38	Subunits
121	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
5	Temperature Range [°C]
35	Temperature Stability [°C]
314	Turnover Number [1/s]

pH Stability

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pH Stability on EC 1.1.3.10 - pyranose oxidase

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

1 - 2

Phlebiopsis gigantea

Q6UG02

pH 7.0, 30 min, wild-type enzyme loses about 75% of its activity, mutant enzyme K312E with a C-terminal His6-tag loses about 70% of its activity and mutant enzyme E540K loses about 10% of its activity

654320

10

Phlebiopsis gigantea

Q6UG02

pH 7.0, 30 min, wild-type enzyme loses about 20% of its activity, mutant enzyme K312E with a C-terminal His6-tag and mutant enzyme E540K are stable

654320

11

2 entries

3

Trametes ochracea

-

half-life: 13 days

389649

3 - 11

Irpex lacteus

-

almost no activity loss occurs in the pH range 5.0-11.0 on incubation for 24 h at 25°C. Nearly half and 80% of its initial activity remains at pH 3.0 and 3.5, respectively

741354

3.5

Trametes ochracea

-

half-life: 52 days

389649

4

2 entries

4 - 4.5

Phanerodontia chrysosporium

-

50 mM acetate, the enzyme is relatively stable

389646

4 - 9

Polyporus obtusus

-

-

389641, 389642

4.5

2 entries

5 - 7

Trametes ochracea

-

extremely stable, half-life more than 500 days

389649

5 - 7.4

Trametes versicolor

-

when kept at 50°C for 30 min

389640

5.5

Tricholoma matsutake

-

37°C, 30 min, about 15% loss of activity

655049

5.5 - 9

Basidiomycota

-

no loss of activity on incubation for 30 min at 50°C

389635

6

2 entries

6 - 7

Phanerodontia chrysosporium

-

bisTris, the enzyme is relatively stable

389646

7 - 11

Tricholoma matsutake

-

37°C, 30 min, stable

655049

7 - 9

Phlebiopsis gigantea

Q6UG02

30 min, wild-type enzyme, mutant enzyme K312E with a C-terminal His6-tag and mutant enzyme E540K are stable

654320

8

2 entries

additional information

Trametes ochracea

-

stability of soluble and immobilized enzymes examined by incubating samples at 30°C for 7 days in citrate-phosphate buffer ranging from pH 4 to 8, similar stability obtained in free or immobilized form

687890

11

Basidiomycota

-

78% of its initial activity remains on incubation for 30 min at 50°C, glycine-NaOH buffer

389635

11

Phlebiopsis gigantea

Q6UG02

pH 7.0, 30 min, wild-type enzyme loses about 40% of its activity, mutant enzyme K312E with a C-terminal His6-tag loses about 10% of its activity, mutant enzyme E540K is stable

654320

4

Basidiomycota

-

67% of its initial activity remains, citrate buffer

389635

4

Trametes ochracea

-

half-life: 130 days

389649

4.5

Trametes ochracea

-

half-life: 350 days

389649

4.5

Tricholoma matsutake

-

37°C, 30 min, 60% loss of activity

655049

6

Phanerodontia chrysosporium

-

2-(N-morpholino)ethane sulfonic acid, the enzyme is relatively stable

389646

6

Phlebiopsis gigantea

Q6UG02

30 min, 30 min, wild-type enzyme and mutant enzyme E540K are stable, mutant enzyme K312E with a C-terminal His6-tag loses about 10% of its activity

654320

8

Phanerodontia chrysosporium

-

optimal stability, 50 mM Tris-HCl, triethanolamine and imidazole, more than 95% of the residual activity remains after 36 days

389646

8

Phanerodontia chrysosporium

-

4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid, the enzyme is relatively stable

389646