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1.1.1.B66: acyclic imino acid reductase

This is an abbreviated version!
For detailed information about acyclic imino acid reductase, go to the full flat file.

Reaction

an acyclic iminoacid
+
NADPH
 +
H+
=
an amino acid
 +
NADP+

Synonyms

asymmetric reductase, BSp5, imino acid reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B66 acyclic imino acid reductase

Engineering

Engineering on EC 1.1.1.B66 - acyclic imino acid reductase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H130A
site-directed mutagenesis, the mutant shows highly reduced activity with substrate (4E)-5-carbamimidamido-2-iminopent-4-enoic acid, but unaltered activity with 5-carbamimidamido-2-iminopentanoic acid compared to wild-type
H130A/Y132F
site-directed mutagenesis, the mutant shows highly reduced activity with substrate (4E)-5-carbamimidamido-2-iminopent-4-enoic acid, but increased activity with 5-carbamimidamido-2-iminopentanoic acid compared to wild-type
R229A
site-directed mutagenesis, the mutant shows reduced activity with substrates (4E)-5-carbamimidamido-2-iminopent-4-enoic acid and 5-carbamimidamido-2-iminopentanoic acid compared to wild-type
Y132F
site-directed mutagenesis, the mutant shows highly reduced activity with substrate (4E)-5-carbamimidamido-2-iminopent-4-enoic acid, but unaltered activity with 5-carbamimidamido-2-iminopentanoic acid compared to wild-type
Y97F
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
additional information
the mutations do not impact dimerization