1.1.1.B66: acyclic imino acid reductase

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For detailed information about acyclic imino acid reductase, go to the full flat file.

Reaction

Synonyms

asymmetric reductase, BSp5, imino acid reductase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.B66 acyclic imino acid reductase

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Results	in table
1	AA Sequence
1	Cloned(Commentary)
2	Cofactor
1	Crystallization (Commentary)
3	General Information
1	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
1	Organism
1	pH Optimum
6	Protein Variants
1	Purification (Commentary)
2	Reaction
1	Reference
4	Substrates and Products (Substrate)
2	Subunits
3	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Crystallization

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Crystallization on EC 1.1.1.B66 - acyclic imino acid reductase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified enzyme Bsp5 in complex with D-arginine and coenzyme NADPH, hanging drop vapour diffusion, mixing of 800 nl of 10 mg/m protein in 20 mM HEPES, 50 mM NaCl, pH 7.5, 5 mM D-Arg, and 5 mM NADPH, with 800 nl of reservoir solution containing 20% w/v PEG 3350 and 0.2 M sodium tartrate dibasic, equilibration against 0.05 ml of reservoir solution, room temperature, 10 days, method optimization, X-ray diffraction structure determination and analysis at 1.6 A resolution, molecular replacement, modeling

Bacillus sp. 5mfcol3.1

A0A1I4FUG4

763186