1.1.1.B4: (R)-specific secondary alcohol dehydrogenase (NADH)

This is an abbreviated version!
For detailed information about (R)-specific secondary alcohol dehydrogenase (NADH), go to the full flat file.

Word Map on EC 1.1.1.B4

Reaction

(R)-R-CHOH-R'
+
NAD+
=
R-CO-R'
+
NADH
+
H+

Synonyms

(R)-epinephrine dehydrogenase, ADH, ADH1, ADH2, ADHTt, carbonyl reductase, carbonyl reductase (NADH, specific for (S)-configuration of alcohol), CHY1186, CpCR, mLDH, More, SADH, SDR, secondary alcohol dehydrogenase, short-chain NAD(H)-dependent alcohol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B4 (R)-specific secondary alcohol dehydrogenase (NADH)

Purification

Purification on EC 1.1.1.B4 - (R)-specific secondary alcohol dehydrogenase (NADH)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 226fold to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by gel filtration
-
native enzyme by a procedure including RedA-agarose affinity chromatography to electrophoretical homogeneity
-
recombinant C-terminally His-tagged mutant I86A from Escherichia coli HB101 (DE3) by nickel affinity chromatography
recombinant enzyme 11fold to homogeneity from Escherichia coli strain BL21(DE3) by heat denaturation of host proteins, anion exchange chromatography and gel filtration
-