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1.1.1.91: aryl-alcohol dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about aryl-alcohol dehydrogenase (NADP+), go to the full flat file.

Reaction

an aromatic alcohol
+
NADP+
=
an aromatic aldehyde
+
NADPH
+
H+

Synonyms

AAD, AAD1, AAD14, Aad14p, Aad1p, AAD4, Aad4p, ADH, aryl alcohol dehydrogenase (nicotinamide adenine dinucleotide phosphate) coniferyl alcohol dehydrogenase, aryl-alcohol dehydrogenase, coniferyl alcohol dehydrogenase, NADPH-linked benzaldehyde reductase, PcAad1p, ScAad14p protein, ScAad4p protein, SDR A5, SDR B3, short-chain dehydrogenase/reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.91 aryl-alcohol dehydrogenase (NADP+)

Cloned

Cloned on EC 1.1.1.91 - aryl-alcohol dehydrogenase (NADP+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Top10 cells
-
expressed in Saccharomyces cerevisiae
gene AAD1, phylogenetic analysis, subcloning of tagged PcAAD4 into the multicopy YEplac195 plasmid under the control of the strong constitutive PGK1 promoter and recombinant expression in Saccharomyces wild-type strain BY4741, activity in BY4741 cell crude extracts expressing PcAad1p is 2fold higher than that of the blank-plasmid control
-
gene AAD14, phylogenetic analysis, subcloning of tagged ScAAD14 into the multicopy YEplac195 plasmid under the control of the strong constitutive PGK1 promoter and recombinant expression in Saccharomyces wild-type strain BY4741, the enzyme activity in cells expressing ScAd14p does not significantly differ from control
gene AAD4, phylogenetic analysis, subcloning of tagged ScAAD4 into the multicopy YEplac195 plasmid under the control of the strong constitutive PGK1 promoter and recombinant expression in Saccharomyces wild-type strain BY4741, the enzyme activity in cells expressing ScAd4p does not significantly differ from control