1.1.1.9: D-xylulose reductase

This is an abbreviated version!
For detailed information about D-xylulose reductase, go to the full flat file.

Word Map on EC 1.1.1.9

Reaction

xylitol
+
NAD+
=
D-xylulose
+
NADH
+
H+

Synonyms

2,3-cis-polyol(DPN) dehydrogenase (C3-5), D-xylulose reductase A, erythritol dehydrogenase, GmXDH, NAD+-dependent XDH, NAD+-dependent xylitol dehydrogenase, NAD+-linked xylitol dehydrogenase, NAD-dependent xylitol dehydrogenase, pentitol-DPN dehydrogenase, PsXDH, reductase, D-xylulose, RpXDH, slSDH, XDH, XDH-Y25, xdhA, XL2, XYL2, xylitol dehydrogenase, xylitol-2-dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.9 D-xylulose reductase

Purification

Purification on EC 1.1.1.9 - D-xylulose reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, DEAE Toyopearl chromatography, phenyl Toyopearl chromatography, Mono Q chromatography, Superdex 200 gel filtration, and Reactive Red column chromatography
-
DEAEToyopearl 650 M column chromatography, butyl-Toyopearl 650 M column chromatography, MonoQ column chromatography, and Superdex 200 gel filtration; native enzyme by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interation chromatography, and another different step of anion exchange chromatography, followed by gel filtration. Recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography
native enzyme 469fold from sstrain NH-10 by ammonium sulfate fractionation, two different steps of anion exchange chromatography, and gel filtration
-
native enzyme from Rhizomucor pusillus strain NBRC 4578 by anion exchange chromatography, ammonium sulfate fractionation, and hydrophobic interaction chromatography, followed by ultrafiltration, and a another different step of anion exchange chromatography, ultrafiltration, and gel filtration. Next purification steps are Reactive Red 120 affinity chromatography, dialysis, and ultrafiltration. Recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
Ni-NTA column chromatography and Sephacryl S-300 gel filtration
-
optimized extraction by cetyl trimethyl ammonium bromide reversed micelles
-
partial
recombinant enzmye
recombinant enzyme
recombinant His6-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration
using affinity chromatography and preparative gel filtration
-
using Ni-NTA chromatography
-