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0.0025 - 220
(2R,3S)-3-isopropylmalate
0.016 - 0.85
3-(2'-methylthio)ethylmalate
3.8 - 14.9
3-isopropylmalate
2.7
3-tert-butylmalate
-
-
3.1
D-3-ethylmalate
pH 8.0
3.1
D-3-isobutylmalate
pH 8.0
2.7
D-3-isopentylmalate
pH 8.0
3.6
D-3-isopropylmalate
pH 8.0
3.5
D-3-methylmalate
pH 8.0
0.96
D-3-t-butylmalate
pH 8.0
0.41
D-malate
-
pH 7.5, 37°C
0.26
homoisocitrate
at pH 8.0 and 60°C
0.09 - 69
isopropylmalate
6
tert-butylmalate
-
pH 7.6
additional information
additional information
-
the cold adaption results from the destabilization of the ternary complex caused by increase in the volume of the residue at position 126
-
0.0025
(2R,3S)-3-isopropylmalate
mutant enzyme D241A, at pH 7.6 and 20°C
0.0025
(2R,3S)-3-isopropylmalate
mutant enzyme K185A, at pH 7.6 and 20°C
0.003
(2R,3S)-3-isopropylmalate
mutant enzyme D292N, at pH 7.5 and 25°C
0.017
(2R,3S)-3-isopropylmalate
mutant enzyme Y181A, at pH 7.5 and 25°C
0.023
(2R,3S)-3-isopropylmalate
mutant enzyme R146K, at pH 7.5 and 25°C
0.03
(2R,3S)-3-isopropylmalate
mutant enzyme R146A, at pH 7.5 and 25°C
0.033
(2R,3S)-3-isopropylmalate
mutant enzyme E270A, at pH 7.6 and 20°C
0.033
(2R,3S)-3-isopropylmalate
mutant enzyme E270A, in the presence of K+, at pH 7.6 and 20°C
0.033
(2R,3S)-3-isopropylmalate
mutant enzyme R136K, at pH 7.5 and 25°C
0.045
(2R,3S)-3-isopropylmalate
mutant enzyme D217A, at pH 7.6 and 20°C
0.08
(2R,3S)-3-isopropylmalate
wild-type HICDH, pH 8.0, 37°C
0.108
(2R,3S)-3-isopropylmalate
mutant enzyme Y139A, at pH 7.6 and 20°C
0.233
(2R,3S)-3-isopropylmalate
mutant enzyme Y181F, at pH 7.5 and 25°C
0.3
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/V238M/R310M, pH 8.0, 37°C
0.4
(2R,3S)-3-isopropylmalate
mutant R85V/Y86A, pH 8.0, 37°C
0.433
(2R,3S)-3-isopropylmalate
mutant enzyme D245A, at pH 7.6 and 20°C
0.47
(2R,3S)-3-isopropylmalate
mutant enzyme L132A, at pH 7.5 and 25°C
0.55
(2R,3S)-3-isopropylmalate
mutant enzyme N102A, at pH 7.6 and 20°C
0.583
(2R,3S)-3-isopropylmalate
mutant enzyme Y181H, at pH 7.5 and 25°C
0.6
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M, pH 8.0, 37°C
0.62
(2R,3S)-3-isopropylmalate
mutant L133F, pH not specified in the publication, temperature not specified in the publication
0.622
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.73
(2R,3S)-3-isopropylmalate
mutant L134F, pH not specified in the publication, temperature not specified in the publication
1.2
(2R,3S)-3-isopropylmalate
-
acIPMDH-ML, pH 8.0, 25°C
1.6
(2R,3S)-3-isopropylmalate
mutant S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
1.62
(2R,3S)-3-isopropylmalate
mutant enzyme R174K, at pH 7.5 and 25°C
1.7
(2R,3S)-3-isopropylmalate
-
ancIPMDH-IQ, pH 8.0, 25°C
1.8
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/R310M, pH 8.0, 37°C
2
(2R,3S)-3-isopropylmalate
mutant enzyme V235A, at pH 7.5 and 25°C
2.36
(2R,3S)-3-isopropylmalate
-
pH 8.0, 40°C, wild-type enzyme
2.41
(2R,3S)-3-isopropylmalate
-
pH 8.0, 40°C, mutant enzyme C275T
2.9
(2R,3S)-3-isopropylmalate
mutant H15Y/E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
2.9
(2R,3S)-3-isopropylmalate
-
pH 7.6, 20°C, quenched flow experiment
3.1
(2R,3S)-3-isopropylmalate
-
pressure 250 MPa, 20°C, pH not specified in the publication
3.1
(2R,3S)-3-isopropylmalate
mutant enzyme L133A, at pH 7.5 and 25°C
3.4
(2R,3S)-3-isopropylmalate
-
pH 7.6, 20°C, stopped flow experiment
3.83
(2R,3S)-3-isopropylmalate
mutant F137L, pH not specified in the publication, temperature not specified in the publication
3.97
(2R,3S)-3-isopropylmalate
wild type enzyme, in the presence of K+, at pH 7.6 and 20°C
4.3
(2R,3S)-3-isopropylmalate
-
acIPMDH-ML, pH 8.0, 40°C
4.57
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.5 and 25°C
4.6
(2R,3S)-3-isopropylmalate
-
ancIPMDH-IQ, pH 8.0, 40°C
5.1
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
5.7
(2R,3S)-3-isopropylmalate
wild-type (2R,3S)-3-isopropylmalate dehydrogenase, pH 8.0, 37°C
5.8
(2R,3S)-3-isopropylmalate
-
pressure 200 MPa, 20°C, pH not specified in the publication
6.22
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
7.2
(2R,3S)-3-isopropylmalate
-
pH 8.0, 40°C, mutant enzyme A31G/G43A/A709G
7.6
(2R,3S)-3-isopropylmalate
-
pressure 100 MPa, 20°C, pH not specified in the publication
8.4
(2R,3S)-3-isopropylmalate
-
pressure 150 MPa, 20°C, pH not specified in the publication
8.5
(2R,3S)-3-isopropylmalate
at pH 8.0 and 60°C
9.05
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
9.6
(2R,3S)-3-isopropylmalate
-
mutant enzyme I268A, at pH 7.6 and 25°C
10
(2R,3S)-3-isopropylmalate
pressure 250 MPa, 20°C, pH not specified in the publication
10.7
(2R,3S)-3-isopropylmalate
-
pH 8.0, 40°C, mutant enzyme G43A
12
(2R,3S)-3-isopropylmalate
-
pH 7.5, 37°C
13
(2R,3S)-3-isopropylmalate
pressure 200 MPa, 20°C, pH not specified in the publication
14
(2R,3S)-3-isopropylmalate
-
pressure 0.1 MPa, 20°C, pH not specified in the publication
14
(2R,3S)-3-isopropylmalate
pressure 100 MPa, 20°C, pH not specified in the publication
14
(2R,3S)-3-isopropylmalate
pressure 150 MPa, 20°C, pH not specified in the publication
14.8
(2R,3S)-3-isopropylmalate
-
mutant enzyme M106L, at pH 7.6 and 25°C
15
(2R,3S)-3-isopropylmalate
pressure 0.1 MPa, 20°C, pH not specified in the publication
15
(2R,3S)-3-isopropylmalate
-
pressure 50 MPa, 20°C, pH not specified in the publication
16.1
(2R,3S)-3-isopropylmalate
mutant enzyme A268I, at pH 7.6 and 25°C
16.4
(2R,3S)-3-isopropylmalate
-
wild type enzyme, at pH 7.6 and 25°C
16.7
(2R,3S)-3-isopropylmalate
mutant enzyme S266A, at pH 7.6 and 25°C
17
(2R,3S)-3-isopropylmalate
pressure 50 MPa, 20°C, pH not specified in the publication
17.4
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.6 and 25°C
17.7
(2R,3S)-3-isopropylmalate
-
pH 8.0, 40°C, mutant enzyme G376A
20.4
(2R,3S)-3-isopropylmalate
-
mutant enzyme A266S, at pH 7.6 and 25°C
22
(2R,3S)-3-isopropylmalate
-
acIPMDH-ML, pH 8.0, 70°C
22
(2R,3S)-3-isopropylmalate
-
ancIPMDH-IQ, pH 8.0, 70°C
28
(2R,3S)-3-isopropylmalate
-
-
40
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.6 and 20°C
44.7
(2R,3S)-3-isopropylmalate
-
pH 8.0, 70°C, mutant enzyme C275T
45.8
(2R,3S)-3-isopropylmalate
-
pH 8.0, 70°C, wild-type enzyme
48.1
(2R,3S)-3-isopropylmalate
mutant enzyme L106M, at pH 7.6 and 25°C
50.1
(2R,3S)-3-isopropylmalate
-
pH 8.0, 70°C, mutant enzyme A31G/G43A/A709G
63.6
(2R,3S)-3-isopropylmalate
-
pH 8.0, 70°C, mutant enzyme G43A
100
(2R,3S)-3-isopropylmalate
-
pH 8.0, 70°C, mutant enzyme G376A
100
(2R,3S)-3-isopropylmalate
-
55°C, mutant enzyme A172D
107
(2R,3S)-3-isopropylmalate
-
55°C, wild-type enzyme
111
(2R,3S)-3-isopropylmalate
-
55°C, mutant enzyme A172Q
117
(2R,3S)-3-isopropylmalate
-
55°C, mutant enzyme A172E and A172N
163
(2R,3S)-3-isopropylmalate
60°C, pH 7.6, wild-type enzyme
220
(2R,3S)-3-isopropylmalate
60°C, pH 7.6, mutant enzyme A172V
0.016
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.0167
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.033
3-(2'-methylthio)ethylmalate
mutant F137L, pH not specified in the publication, temperature not specified in the publication
0.27
3-(2'-methylthio)ethylmalate
mutant L134F, pH not specified in the publication, temperature not specified in the publication
0.367
3-(2'-methylthio)ethylmalate
mutant L133F, pH not specified in the publication, temperature not specified in the publication
0.85
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
3.8
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant F3S/S232G
6
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant R284G
6.8
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant F3S
6.9
3-isopropylmalate
pH 7.5, 70°C, recombinant wild-type enzyme
7.5
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant L288P
7.8
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant F3S/L288P
8.3
3-isopropylmalate
-
mutant Glu253Trp
9.5
3-isopropylmalate
-
mutant Glu253Ala
9.9
3-isopropylmalate
-
mutant Glu253Gln
12.4
3-isopropylmalate
-
mutant Glu253Val
13.7
3-isopropylmalate
-
mutant Glu253Leu
14.9
3-isopropylmalate
-
wild-type
0.09
isopropylmalate
-
pH 7.3, 21°C, reaction with NADP+, mutant enzyme S226R/D278K/I279Y
0.15
isopropylmalate
-
pH 7.3, 21°C, reaction with NAD+, wild-type enzyme
0.2
isopropylmalate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G
0.21
isopropylmalate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q
0.24
isopropylmalate
-
pH 7.3, 21°C, reaction with NAD+, wild-type enzyme
0.26
isopropylmalate
-
pH 7.3, 21°C, reaction with NADP+, wild-type enzyme
0.48
isopropylmalate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme S226R
0.88
isopropylmalate
-
pH 7.3, 21°C, reaction with NADP+, mutant enzyme S226R
1.66
isopropylmalate
-
pH 7.3, 21°C, reaction with NADP+, wild-type enzyme
1.91
isopropylmalate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme S226R/D278K/I279Y
8
isopropylmalate
-
pH 7.6, 70°C
19
isopropylmalate
-
pH 7.6, 40°C
69
isopropylmalate
-
pH 7.6, 40°C
0.19
malate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q
0.26
malate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G
4.69
malate
-
pH 7.3, 21°C, reaction with NADP+, wild-type enzyme
10.6
malate
-
pH 7.3, 21°C, reaction with NAD+, wild-type enzyme
0.15
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme
0.19
NAD+
-
pH 7.3, 21°C, reaction with tartrate, wild-type enzyme or reaction with malate, mutant enzyme E87Q
0.2
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87G
0.21
NAD+
-
pH 7.3, 21°C, reaction with tartrate, mutant enzyme E87G
0.21
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87Q
0.24
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme
0.26
NAD+
-
pH 7.3, 21°C, reaction with malate, mutant enzyme E87G or reaction with tartrate, mutant enzyme E87Q
0.37
NAD+
pH 8.0, 25°C, wild-type enzyme
0.48
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R
1.2
NAD+
-
pH 8.0, 40°C, mutant V126A
1.2
NAD+
-
acIPMDH-ML, pH 8.0, 25°C
1.5
NAD+
-
pH 8.0, 40°C, mutant V126S
1.7
NAD+
-
pH 8.0, 40°C, mutant V126G
1.7
NAD+
-
ancIPMDH-IQ, pH 8.0, 25°C
1.9
NAD+
-
pH 8.0, 40°C, mutant V126T
1.91
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R/D278K/I279Y
2 - 3.7
NAD+
-
mutant enzyme A266S, at pH 7.6 and 25°C
2.36
NAD+
-
pH 8.0, 40°C, wild-type enzyme
2.4
NAD+
pH 8.0, 40°C, wild-type enzyme
2.4
NAD+
-
pH 8.0, 40°C, wild-type
2.41
NAD+
-
pH 8.0, 40°C, mutant enzyme C275T
3.5
NAD+
-
pH 8.0, 40°C, mutant V126E
4.3
NAD+
-
acIPMDH-ML, pH 8.0, 40°C
4.6
NAD+
-
ancIPMDH-IQ, pH 8.0, 40°C
6
NAD+
-
pH 8.0, 40°C, mutant V126I
7.2
NAD+
-
pH 8.0, 40°C, mutant enzyme A31G/G43A/A709G
8
NAD+
-
pH 7.6, 70°C, reaction with isopropylmalate
8.5
NAD+
-
pH 8.0, 40°C, mutant V126L
9
NAD+
-
pH 8.0, 60°C, mutant V126A
10
NAD+
-
pH 8.0, 60°C, V126G
10.2
NAD+
pH 7.6, 70°C, recombinant mutant V181T
10.3
NAD+
-
mutant enzyme I268A, at pH 7.6 and 25°C
10.6
NAD+
-
pH 7.3, 21°C, reaction with malate, wild-type enzyme
10.7
NAD+
-
pH 8.0, 40°C, mutant enzyme G43A
11
NAD+
-
pH 8.0, 40°C, mutant V126F
11.2
NAD+
pH 7.6, 70°C, recombinant mutant D184H
13
NAD+
-
pH 8.0, 60°C, mutant V126S
13.2
NAD+
pH 7.6, 70°C, recombinant mutant V181T/P324T/A335E
13.4
NAD+
pH 7.6, 70°C, recombinant wild-type enzyme
13.8
NAD+
pH 7.6, 70°C, recombinant mutant P324T
14.6
NAD+
pH 7.6, 70°C, recombinant mutant P56E
15
NAD+
-
pH 8.0, 60°C, mutant V126T
15
NAD+
pH 7.6, 70°C, recombinant mutants F53L and V61I
15.2
NAD+
-
mutant enzyme M106L, at pH 7.6 and 25°C
15.6
NAD+
mutant enzyme S266A, at pH 7.6 and 25°C
17.1
NAD+
wild type enzyme, at pH 7.6 and 25°C
17.4
NAD+
mutant enzyme A268I, at pH 7.6 and 25°C
17.7
NAD+
-
pH 8.0, 40°C, mutant enzyme G376A
17.7
NAD+
pH 7.6, 70°C, recombinant mutant R58L
18
NAD+
-
pH 8.0, 40°C, mutant V126M
18.2
NAD+
pH 7.6, 70°C, recombinant mutants S261N and A335E
18.7
NAD+
-
wild type enzyme, at pH 7.6 and 25°C
19
NAD+
-
pH 7.6, 40°C, reaction with isopropylmalate
20
NAD+
-
pH 8.0, 60°C, wild-type
21
NAD+
-
pH 8.0, 60°C, mutant V126E
21.3
NAD+
pH 7.6, 70°C, recombinant mutant H197K
22
NAD+
-
acIPMDH-ML, pH 8.0, 70°C
22
NAD+
-
ancIPMDH-IQ, pH 8.0, 70°C
25.2
NAD+
pH 7.6, 70°C, recombinant mutant L134N/V181T/P324T/A335E
29.9
NAD+
pH 7.6, 70°C, recombinant mutant L134N
30.5
NAD+
-
pH 8.0, 60°C, mutant V126I
42
NAD+
-
pH 8.0, 60°C, mutant V126F
44.5
NAD+
-
pH 8.0, 60°C, mutant V126L
44.7
NAD+
-
pH 8.0, 70°C, mutant enzyme C275T
45.8
NAD+
-
pH 8.0, 70°C, wild-type enzyme
50.1
NAD+
-
pH 8.0, 70°C, mutant enzyme A31G/G43A/A709G
50.5
NAD+
mutant enzyme L106M, at pH 7.6 and 25°C
54
NAD+
-
pH 8.0, 60°C, mutant V126M
63.6
NAD+
-
pH 8.0, 70°C, mutant enzyme G43A
69
NAD+
-
pH 7.6, 40°C, reaction with isopropylmalate
79
NAD+
pH 8.0, 70°C, wild-type enzyme
100
NAD+
-
pH 8.0, 70°C, mutant enzyme G376A
100
NAD+
-
55°C, mutant enzyme A172D
107
NAD+
-
55°C, wild-type enzyme
111
NAD+
-
55°C, mutant enzyme A172Q
117
NAD+
-
55°C, mutant enzyme A172E and A172N
163
NAD+
60°C, pH 7.6, wild-type enzyme
220
NAD+
60°C, pH 7.6, pH 7.6, mutant enzyme A172V
0.09
NADP+
-
pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R/D278K/I279Y
0.1
NADP+
-
pH 7.3, 21°C, reaction with tartrate, wild-type enzyme
0.26
NADP+
-
pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme
0.88
NADP+
-
pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R
1.66
NADP+
-
pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme
4.69
NADP+
-
pH 7.3, 21°C, reaction with malate, wild-type enzyme
0.1
Tartrate
-
pH 7.3, 21°C, reaction with NADP+, wild-type enzyme
0.19
Tartrate
-
pH 7.3, 21°C, reaction with NAD+, wild-type enzyme
0.21
Tartrate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G
0.26
Tartrate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q