1.1.1.85: 3-isopropylmalate dehydrogenase This is an abbreviated version! For detailed information about 3-isopropylmalate dehydrogenase, go to the full flat file .
Reaction
(2R,3S)-3-isopropylmalate +
NAD+ =
(2S)-2-isopropyl-3-oxosuccinate +
NADH +
H+
Synonyms 2-hydroxy-4-methyl-3-carboxyvalerate:NAD+ oxidoreductase, 2R,3S-isopropylmalate:NAD+ oxidoreductase (decaboxylating), 3-IPM dehydrogenase, 3-IPM-DH, 3-isopropylmalate dehydrogenase, 3-isopropylmalateDH, APS IPMDH, beta-IPM dehydrogenase, beta-IPMDH, beta-isopropylmalate dehydrogenase, beta-isopropylmalic enzyme, Bs3-isopropylmalateDH, dehydrogenase, 3-isopropylmalate, IMDH, IPMDH, IPMDH1, IPMDH2, IPMDH3, isopropylmalate dehydrogenase, leuB, NAD-dependent isopropylmalate dehydrogenase, Saci_0600, SbIPMDH, SoIPMDH, threo-Ds-3-isopropylmalate dehydrogenase, Tt-beta-3-isopropylmalateDH, two-domain 3-isopropylmalate dehydrogenase, Ydr417cp
ECTree
Specific Activity
Specific Activity on EC 1.1.1.85 - 3-isopropylmalate dehydrogenase
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0.54
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slow electrophoretic form
1.87
-
acIPMDH-ML, pH 8.0, 25°C
2.76
-
ancIPMDH-IQ, pH 8.0, 25°C
38.5
-
ancIPMDH-IQ, pH 8.0, 70°C
39.1
-
acIPMDH-ML, pH 8.0, 70°C
19.4
-
pH 7.0, 55°C
7.6
-
mutant E253F
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radiochemical assay
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the specific activities of ancIPMDH-IQ and ancIPMDHML are, respectively, 3.1fold and 2.1fold greater than that of Thermus thermophilus IPMDH at 25°C. At 70°C, the specificity activities of the ancestral IPMDHs are greater than those of the mesophilic IPMDHs but smaller than that of the thermophilic enzyme
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