1.1.1.85: 3-isopropylmalate dehydrogenase
This is an abbreviated version!
For detailed information about 3-isopropylmalate dehydrogenase, go to the full flat file.
Word Map on EC 1.1.1.85
Reaction
Synonyms
2-hydroxy-4-methyl-3-carboxyvalerate:NAD+ oxidoreductase, 2R,3S-isopropylmalate:NAD+ oxidoreductase (decaboxylating), 3-IPM dehydrogenase, 3-IPM-DH, 3-isopropylmalate dehydrogenase, 3-isopropylmalateDH, APS IPMDH, beta-IPM dehydrogenase, beta-IPMDH, beta-isopropylmalate dehydrogenase, beta-isopropylmalic enzyme, Bs3-isopropylmalateDH, dehydrogenase, 3-isopropylmalate, IMDH, IPMDH, IPMDH1, IPMDH2, IPMDH3, isopropylmalate dehydrogenase, leuB, NAD-dependent isopropylmalate dehydrogenase, Saci_0600, SbIPMDH, SoIPMDH, threo-Ds-3-isopropylmalate dehydrogenase, Tt-beta-3-isopropylmalateDH, two-domain 3-isopropylmalate dehydrogenase, Ydr417cp
ECTree
1.1.1.85 3-isopropylmalate dehydrogenaseAdvanced search results
results (
results (KM Value
KM Value on EC 1.1.1.85 - 3-isopropylmalate dehydrogenase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.0035
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.5 and 25°C
0.0063
(2R,3S)-3-isopropylmalate
mutant enzyme Y181H, at pH 7.5 and 25°C
0.0074
(2R,3S)-3-isopropylmalate
mutant enzyme Y181F, at pH 7.5 and 25°C
0.0083
(2R,3S)-3-isopropylmalate
mutant enzyme Y181A, at pH 7.5 and 25°C
0.0087
(2R,3S)-3-isopropylmalate
-
pressure 100 MPa, 20°C, pH not specified in the publication
0.0092
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.0109
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.011
(2R,3S)-3-isopropylmalate
mutant F137L, pH not specified in the publication, temperature not specified in the publication
0.0114
(2R,3S)-3-isopropylmalate
mutant enzyme V235A, at pH 7.5 and 25°C
0.0122
(2R,3S)-3-isopropylmalate
mutant enzyme L106M, at pH 7.6 and 25°C
0.013
(2R,3S)-3-isopropylmalate
mutant enzyme D217A, at pH 7.6 and 20°C
0.014
(2R,3S)-3-isopropylmalate
mutant enzyme N102A, at pH 7.6 and 20°C
0.0141
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.6 and 25°C
0.015
(2R,3S)-3-isopropylmalate
-
pressure 0.1 MPa, 20°C, pH not specified in the publication
0.016
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.6 and 20°C
0.016
(2R,3S)-3-isopropylmalate
wild type enzyme, in the presence of 25 mM K+, at pH 7.6 and 20°C
0.0169
(2R,3S)-3-isopropylmalate
-
mutant enzyme A172D, at 55°C, pH not specified in the publication
0.019
(2R,3S)-3-isopropylmalate
-
pH 9.0
0.02
(2R,3S)-3-isopropylmalate
pressure 100 MPa, 20°C, pH not specified in the publication
0.02
(2R,3S)-3-isopropylmalate
mutant enzyme D245A, at pH 7.6 and 20°C
0.022
(2R,3S)-3-isopropylmalate
60°C, pH 7.6, mutant enzyme A172V
0.0221
(2R,3S)-3-isopropylmalate
-
wild type enzyme, at 55°C, pH not specified in the publication
0.0226
(2R,3S)-3-isopropylmalate
mutant enzyme S266A, at pH 7.6 and 25°C
0.0234
(2R,3S)-3-isopropylmalate
-
mutant enzyme A172N, at 55°C, pH not specified in the publication
0.0239
(2R,3S)-3-isopropylmalate
-
mutant enzyme M106L, at pH 7.6 and 25°C
0.0242
(2R,3S)-3-isopropylmalate
mutant enzyme L132A, at pH 7.5 and 25°C
0.025
(2R,3S)-3-isopropylmalate
pressure 0.1 MPa, 20°C, pH not specified in the publication
0.025
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.0257
(2R,3S)-3-isopropylmalate
-
mutant enzyme A172E, at 55°C, pH not specified in the publication
0.0267
(2R,3S)-3-isopropylmalate
-
mutant enzyme A172Q, at 55°C, pH not specified in the publication
0.027
(2R,3S)-3-isopropylmalate
-
pressure 50 MPa, 20°C, pH not specified in the publication
0.0278
(2R,3S)-3-isopropylmalate
mutant enzyme D292N, at pH 7.5 and 25°C
0.028
(2R,3S)-3-isopropylmalate
mutant L134F, pH not specified in the publication, temperature not specified in the publication
0.0303
(2R,3S)-3-isopropylmalate
mutant L133F, pH not specified in the publication, temperature not specified in the publication
0.032
(2R,3S)-3-isopropylmalate
pressure 150 MPa, 20°C, pH not specified in the publication
0.032
(2R,3S)-3-isopropylmalate
mutant enzyme E270A, at pH 7.6 and 20°C
0.032
(2R,3S)-3-isopropylmalate
mutant enzyme E270A, in the presence of 25 mM K+, at pH 7.6 and 20°C
0.0344
(2R,3S)-3-isopropylmalate
-
mutant enzyme A266S, at pH 7.6 and 25°C
0.0345
(2R,3S)-3-isopropylmalate
mutant enzyme A268I, at pH 7.6 and 25°C
0.0356
(2R,3S)-3-isopropylmalate
-
mutant enzyme I268A, at pH 7.6 and 25°C
0.038
(2R,3S)-3-isopropylmalate
pressure 200 MPa, 20°C, pH not specified in the publication
0.04
(2R,3S)-3-isopropylmalate
mutant enzyme Y139A, at pH 7.6 and 20°C
0.043
(2R,3S)-3-isopropylmalate
pressure 50 MPa, 20°C, pH not specified in the publication
0.053
(2R,3S)-3-isopropylmalate
-
pressure 150 MPa, 20°C, pH not specified in the publication
0.055
(2R,3S)-3-isopropylmalate
-
pressure 200 MPa, 20°C, pH not specified in the publication
0.0707
(2R,3S)-3-isopropylmalate
mutant enzyme R146A, at pH 7.5 and 25°C
0.092
(2R,3S)-3-isopropylmalate
-
pressure 250 MPa, 20°C, pH not specified in the publication
0.108
(2R,3S)-3-isopropylmalate
pressure 250 MPa, 20°C, pH not specified in the publication
0.119
(2R,3S)-3-isopropylmalate
mutant enzyme R146K, at pH 7.5 and 25°C
0.125
(2R,3S)-3-isopropylmalate
mutant enzyme L133A, at pH 7.5 and 25°C
0.145
(2R,3S)-3-isopropylmalate
mutant enzyme K185A, at pH 7.6 and 20°C
0.255
(2R,3S)-3-isopropylmalate
mutant enzyme D241A, at pH 7.6 and 20°C
0.59
(2R,3S)-3-isopropylmalate
mutant H15Y/E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
0.753
(2R,3S)-3-isopropylmalate
mutant enzyme R174K, at pH 7.5 and 25°C
0.9
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
0.9
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/V238M/R310M, pH 8.0, 37°C
1
(2R,3S)-3-isopropylmalate
mutant S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
1
(2R,3S)-3-isopropylmalate
wild-type (2R,3S)-3-isopropylmalate dehydrogenase, pH 8.0, 37°C
1.5
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M, pH 8.0, 37°C
1.9
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/R310M, pH 8.0, 37°C
3.73
(2R,3S)-3-isopropylmalate
mutant enzyme R136K, at pH 7.5 and 25°C
0.045
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.077
3-(2'-methylthio)ethylmalate
mutant L133F, pH not specified in the publication, temperature not specified in the publication
0.103
3-(2'-methylthio)ethylmalate
mutant L134F, pH not specified in the publication, temperature not specified in the publication
0.323
3-(2'-methylthio)ethylmalate
mutant F137L, pH not specified in the publication, temperature not specified in the publication
0.436
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.502
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.0039
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant F3S/S232G
0.0044
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant L288P
0.0055
3-isopropylmalate
pH 7.5, 70°C, recombinant wild-type enzyme
0.0067
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant F3S/L288P
0.008
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant R284G
0.000017
isopropylmalate
-
pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme
0.00048
isopropylmalate
-
pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87G
0.00298
isopropylmalate
-
pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme
0.0053
isopropylmalate
-
pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87Q
0.0326
malate
-
pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87Q
35.59
malate
-
pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87G
0.00321
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme
0.0149
NAD+
-
pH 8.0, 40°C, reaction with 3-D-isopropylmalate, mutant enzyme C275T
0.225
NAD+
-
pH 8.0, 40°C, isopropylmalate dehydrogenase cold-adapted mutant T1155
0.255
NAD+
pH 7.6, 70°C, recombinant mutant L134N/V181T/P324T/A335E
0.29
NAD+
wild type enzyme, in the presence of 25 mM K+, at pH 7.6 and 20°C
0.39
NAD+
pH 7.5, 70°C, recombinant wild-type enzyme and mutant F3S
0.66
NAD+
mutant enzyme E270A, in the presence of 25 mM K+, at pH 7.6 and 20°C
0.9
NAD+
mutant E57V/S72I/R85M/Y86A/M208T/V238M/R310M, pH 8.0, 37°C
1.01
NAD+
-
at 40°C, isopropylmalate dehydrogenase cold-adapted mutant P215
1.2
NAD+
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
1.2
NAD+
mutant H15Y/E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
1.6
NAD+
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M, pH 8.0, 37°C
1.6
NAD+
mutant S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
1.7
NAD+
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/R310M, pH 8.0, 37°C
1.9
NAD+
wild-type (2R,3S)-3-isopropylmalate dehydrogenase, pH 8.0, 37°C
3.56
NAD+
-
70°C, isopropylmalate dehydrogenase cold-adapted mutant P215
0.02
NADP+
-
pH 7.3, 21°C, mutant enzyme D278K, I279T, P325Y and 395-Arg
1.836
NADP+
-
pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme
0.761
Tartrate
-
pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87Q
0.0061
threo-D,L-isopropylmalate
pH 7.6, 70°C, recombinant wild-type enzyme
0.0063
threo-D,L-isopropylmalate
pH 7.6, 70°C, recombinant mutant V181T/P324T/A335E
0.007
threo-D,L-isopropylmalate
pH 7.6, 70°C, recombinant mutant L134N
0.0161
threo-D,L-isopropylmalate
pH 7.6, 70°C, recombinant mutant L134N/V181T/P324T/A335E
additional information
additional information
-
the Km-value for NAD+ ranges from 0.054 mM to 0.15 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml, the Km-value for 3-isopropylmalate ranges from 0.023 mM to 0.042 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml
-
additional information
additional information
-
the Km-value for NAD+ ranges from 0.054 mM to 0.15 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml, the Km-value for 3-isopropylmalate ranges from 0.023 mM to 0.042 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml
-
additional information
additional information
Michaelis-Menten kinetics of recombinant wild-type and mutant enzymes at different temperatures, overview
-
additional information
additional information
-
Michaelis-Menten kinetics of recombinant wild-type and mutant enzymes at different temperatures, overview
-
additional information
additional information
-
thermodynamic and kinetics analysis, overview
-
