1.1.1.82: malate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about malate dehydrogenase (NADP+), go to the full flat file.

Reaction

Synonyms

(S)-malate dehydrogenase, dehydrogenase, malate (nicotinamide adenine dinucleotide phosphate), L-malate:NAD oxidoreductase, malate NADP dehydrogenase, malic dehydrogenase (nicotinamide adenine dinucleotide phosphate), MDH, NADP malate dehydrogenase, NADP+-dependent malate dehydrogenase, NADP-dependent malate dehydrogenase, NADP-linked malate dehydrogenase, NADP-malate dehydrogenase, NADP-malic enzyme, NADP-MDH, NADP-MDH1, NADP-MDH2, NADPH-MDH

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.82 malate dehydrogenase (NADP+)

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Results	in table
49	Activating Compound
445	AA Sequence
1	Application
1	CAS Registry Number
12	Cloned(Commentary)
27	Cofactor
4	Crystallization (Commentary)
8	Expression
24	General Information
3	General Stability
30	Inhibitors
125	KM Value [mM]
39	Localization
3	Metals/Ions
41	Molecular Weight [Da]
41	Natural Substrates/ Products (Substrates)
116	Organism
1	Oxidation Stability
10	Pathway
5	PDB ID
13	pH Optimum
1	pH Range
1	pH Stability
1	Posttranslational Modification
38	Protein Variants
14	Purification (Commentary)
2	Reaction
3	Reaction Type
107	Reference
2	Renatured (Commentary)
43	Source Tissue
8	Specific Activity [micromol/min/mg]
1	Storage Stability
139	Substrates and Products (Substrate)
34	Subunits
100	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Range [°C]
7	Temperature Stability [°C]
12	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 1.1.1.82 - malate dehydrogenase (NADP+)

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

110000

Aeropyrum pernix

Q9YEA1

recombinant enzyme, gel filtration

696461

110000 - 120000

Zea mays

-

unactivated enzyme, gel filtration

287386

135000

Cicer arietinum

-

gel filtration

287420

143000 - 150000

Zea mays

-

activated enzyme, gel filtration

287386

150000

Zea mays

-

gel filtration

287399

163000

Pisum sativum

-

tetramer, gel filtration

287409

28400

Spinacia oleracea

-

2 * 28400, equilibrium sedimentation of the enzyme denatured by 6 M guanidinium chloride, pH 8, and 30 mM 2-mercaptoethanol

287410

33000

Sorghum sp.

-

x * 33000, DELTAN, a truncation mutant corresponding to the deletion of the 5 end of the mdh cDNA open reading frame until the 73rd codon, SDS-PAGE

287421

33489

Aeropyrum pernix

Q9YEA1

4 * 34000, recombinant enzyme, SDS-PAGE, 4 * 33489, sequence calculation

696461

34000

Aeropyrum pernix

Q9YEA1

4 * 34000, recombinant enzyme, SDS-PAGE, 4 * 33489, sequence calculation

696461

36000

Pisum sativum

-

2 * 36000, mutant DELTAN, lacking the N-terminal 45 residues, SDS-PAGE

287419

38000

Pisum sativum

-

2 * 38000, SDS-PAGE

287418

38500

3 entries

38900

Pisum sativum

-

4 * 38900, native enzyme, SDS-PAGE

287392

39000

Zea mays

-

2 * 39000, SDS-PAGE

287401

40000

2 entries

40306

Echinochloa crus-galli

-

2 * 40306, enzyme from population Quebec, SDS-PAGE

287387

41177

Echinochloa crus-galli

-

2 * 41177, enzyme from population Mississippi, SDS-PAGE

287387

42000

Pisum sativum

-

2 * 42000, wild-type enzyme and mutant enzymes C23A, C28A, C23A/C28A, C23A/C28A/C206A, C23A/C28A/C364A, C23A/C28A/C376A, C23A/C28A/C206A/C364A, C23A/C28A/C206A/C376A and C23A/C28A/C364A/C376A, SDS-PAGE

287419

42207

Sorghum bicolor

-

x * 42207, calculation from nucleotide sequence

287413

43000

2 entries

50000 - 60000

Zea mays

-

unactivated enzyme, gel filtration

287411

55000

Spinacia oleracea

-

equilibrium sedimentation

287401

55680

Spinacia oleracea

-

equilibrium sedimentation

287410

56000

Spinacia oleracea

-

gel filtration

287410

57000

Spinacia oleracea

-

equilibrium sedimentation

287403

61000

Zea mays

-

equilibrium sedimentation

287401

72000

Pisum sativum

-

gel filtration

287418

81000

Zea mays

-

gel filtration

287401

83500

Echinochloa crus-galli

-

enzyme from population Quebec, non-denaturing PAGE

287387

85450

Echinochloa crus-galli

-

isoenzyme 1 and 2 from Mississippi population, non-denaturing PAGE

287387

86700

Echinochloa crus-galli

-

isoenzyme 3 from Mississippi population, non-denaturing PAGE

287387

87000

Pisum sativum

-

dimer, gel filtration

287409

88000

Zea mays

-

gradient pore-PAGE

287393

91000

Zea mays

-

gel filtration

287393

98000

Zea mays

-

gel filtration

287401

additional information

Zea mays

-

-

287399

38500

Pisum sativum

-

1 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE

287409

38500

Pisum sativum

-

2 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE

287409

38500

Pisum sativum

-

4 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE

287409

40000

Pisum sativum

-

monomer, gel filtration

287409

40000

Sorghum sp.

-

x * 40000, wild-type enzyme and mutant enzymes C64S, C69S, C64S/C69S, SDS-PAGE

287421

43000

Zea mays

-

2 * 43000, SDS-PAGE

287393

43000

Zea mays

-

2 * 43000, unactivated dark enzyme form and activated light enzyme form, SDS-PAGE

287386