1.1.1.50: 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)
This is an abbreviated version!
For detailed information about 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific), go to the full flat file.
Word Map on EC 1.1.1.50
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1.1.1.50
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aldo-keto
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androgen
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testosterone
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reductases
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beta-hydroxysteroids
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alpha-reductase
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beta-diol
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androsterone
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dihydrodiol
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3alpha-hydroxysteroids
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dihydrotestosterone
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hydroxysteroids
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alpha-dihydrotestosterone
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alpha-androstane-3
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3-ketosteroids
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alpha-diol
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trans-dihydrodiols
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alpha-androstanediol
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alpha-hsdh
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15-hydroxyprostaglandin
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beta-reductase
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talalay
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bile-acids
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pre-receptor
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akr1d1
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9,10-phenanthrenequinone
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ketosteroid
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alpha-hydroxy-5
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20-ketosteroid
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alpha,17
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alpha-reduced
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chlordecone
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17beta-hsd
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alpha-dht
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environmental protection
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drug development
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biotechnology
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analysis
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medicine
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synthesis
- 1.1.1.50
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aldo-keto
- androgen
- testosterone
- reductases
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beta-hydroxysteroids
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alpha-reductase
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beta-diol
- androsterone
- dihydrodiol
- 3alpha-hydroxysteroids
- dihydrotestosterone
- hydroxysteroids
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alpha-dihydrotestosterone
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alpha-androstane-3
- 3-ketosteroids
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alpha-diol
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trans-dihydrodiols
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alpha-androstanediol
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alpha-hsdh
- 15-hydroxyprostaglandin
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beta-reductase
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talalay
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bile-acids
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pre-receptor
- akr1d1
- 9,10-phenanthrenequinone
- ketosteroid
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alpha-hydroxy-5
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20-ketosteroid
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alpha,17
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alpha-reduced
- chlordecone
- 17beta-hsd
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alpha-dht
- environmental protection
- drug development
- biotechnology
- analysis
- medicine
- synthesis
Reaction
Synonyms
3 alpha-hydroxysteroid dehydrogenase, 3 alphaHD, 3-alpha hydroxysteroid dehydrogenase type 3, 3-alpha-HSD, 3alpha-HSD, 3alpha-HSD/CR, 3alpha-HSD3, 3alpha-HSOR, 3alpha-hydroxysteroid dehydrogenase, 3alpha-hydroxysteroid dehydrogenase type 3, 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase, 3alpha-hydroxysteroid oxido-reductase, 3alpha-hydroxysteroid oxidoreductase, 3alpha-hydroxysteroid-5beta-oxidoreductase, 3alpha-OR, 3alpha-oxidoreductase, 3alpha/3beta-hydroxysteroid dehydrogenase, 3alphaHSD, 3HSD, 5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase, AKR1C, AKR1C1, AKR1C1-4, AKR1C14, AKR1C17, AKR1C2, AKR1C3, AKR1C4, AKR1C9, aldo-keto reductase 1C2, bile-acid binding protein, chlordecone reductase, DD2, DD21, dihydrodiol dehydrogenase, HSD, HSD28, HSD29, hsdA, HSDH, hydroxyprostaglandin dehydrogenase, More, NAD(P)+-3alpha-hydroxysteroid dehydrogenase, NAD+-dependent 3alpha-HSD, NADP(H)-dependent 3alpha-HSD, NADPH:5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase, prostaglandin F2alpha-synthase, Ps3alphaHSD, PT3HSD, sterognost 3alpha dehydrogenase, 3alpha-hydroxy steroid, type 2 3alpha-hydroxysteroid dehydrogenase/type 5 17beta-hydroxysteroid dehydrogenase, type 3 3alpha-hydroxysteroid dehydrogenase, type 5 17beta-hydroxysteroid dehydrogenase, type I 3alpha-HSD
ECTree
1.1.1.50 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)Advanced search results
results (
results (KM Value
KM Value on EC 1.1.1.50 - 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.017 - 0.042
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
0.069
(3alpha,5beta,7beta)-3,7-dihydroxycholan-24-oic acid
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pH 8.5, 37°C, with cofactor NAD+
0.0027
(5beta,20R)-20-hydroxypregnan-3-one
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pH 7.4, 25°C, recombinant wild-type AKR1C17
0.2 - 0.8
4-Nitroacetophenone
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also in this range: 2,3-hexanedione, 1-phenyl-1,2-propanedione, 2,3-pentanedione, pyridine-4-aldehyde, 2,3-butanedione
0.235
5beta-Pregnan-3alpha-ol-20-one
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cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
0.0024
5beta-pregnane-3,20-dione
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pH 7.4, 25°C, recombinant wild-type AKR1C17
0.023
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one
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pH 7.4, 25°C, recombinant wild-type AKR1C17
0.003
9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one
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recombinant enzyme in intact COS-1 cells
0.004
Dehydrolithocholic acid
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pH 7.4, 25°C, recombinant wild-type AKR1C17
0.00315
dihydrotestosterone
37°C, pH not specified in the publication
0.0025
oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one
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isozyme AKR1C3
0.017
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
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NADP+, detection spectrophotometrically
0.019
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
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NADP+, detection radio- or fluorometrically
0.022
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
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pH 7.0, 37°C with NADP+
0.042
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
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pH 7.0, 37°C with NAD+
0.022
1-Acenaphthenol
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pH 7.4, 25°C, recombinant isozyme AKR1C1 and isozyme AKR1C2
0.002 - 0.0024
17beta-hydroxy-5alpha-androstan-3-one
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also in this range: 3alpha,7alpha-dihydroxy-5beta-cholanoyl glycine
0.0012 - 0.0017
17beta-hydroxy-5beta-androstan-3-one
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also in this range: 5beta-dihydrotestosterone, rat epididymis
0.0012 - 0.0017
17beta-hydroxy-5beta-androstan-3-one
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also in this range: 17alpha,21-dihydroxy-5beta-pregnan-3,11,20-trione, acenaphthequinone
0.0012
5alpha-androstan-3,17-dione
wild type enzyme, in 0.1 M HEPES at pH 7.5
0.0031
5alpha-androstan-3,17-dione
mutant enzyme S114A, in 0.1 M HEPES at pH 7.5
0.00218
5alpha-dihydrotestosterone
pH 7.4, 37°C, recombinant wild-type enzyme
0.007 - 0.0083
5alpha-dihydrotestosterone
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also in this range: androstanedione
0.007 - 0.0083
5alpha-dihydrotestosterone
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also in this range: 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine
0.007 - 0.0083
5alpha-dihydrotestosterone
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also in this range: 3alpha-hydroxy-5alpha-androstan-17-one
0.007 - 0.0083
5alpha-dihydrotestosterone
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also in this range: 3alpha-hydroxy-5beta-pregnan-17-one
0.00035 - 0.00049
5beta-androstan-3,17-dione
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also in this range: 5beta-pregnan-3,20-dione, 5alpha-androstan-3,17-dione
0.0021
5beta-androstane-3,17-dione
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pH 7.4, 25°C, recombinant wild-type AKR1C17
0.038
9,10-phenanthrenequinone
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pH 7.4, 25°C, recombinant wild-type AKR1C17
0.00044
androsterone
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pH 9.0, 25°C, recombinant His-tagged mutant S114A/Y155F
0.0035
androsterone
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pH 7.4, 25°C, recombinant isozyme AKR1C2 and isozyme AKR1C4
0.0051
androsterone
pH 10.4, 25°C, recombinant wild-type enzyme
0.007
androsterone
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pH 9.0, 25°C, recombinant His-tagged mutant S114A
0.065
androsterone
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pH 9.0, 25°C, recombinant His-tagged mutant K159A
0.074
androsterone
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pH 9.0, 25°C, recombinant His-tagged mutant Y155F
0.1
androsterone
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pH 9.0, 25°C, recombinant His-tagged mutant Y155F/K159A
0.01 - 0.025
cholic acid
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3alpha-hydroxy-5beta-androstan-17-one, 3alpha,21-dihydroxy-5beta-pregnan-20-one, 5beta-androstan-3alpha,17beta-diol, 5alpha-androstan-3alpha,17beta-diol, NADP+
0.01 - 0.025
cholic acid
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NAD+, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine
0.0052 - 0.0059
glycolithocholic acid
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also in this range: 3alpha-hydroxy-5alpha-pregnan-20-one
0.0038
NAD+
wild type enzyme, in 0.1 M CAPS buffer, at pH 10.2, at 25°C
0.005
NAD+
mutant enzyme D249S, in 0.1 M CAPS buffer, at pH 10.2, at 25°C
0.009
NAD+
mutant enzyme D249K, in 0.1 M CAPS buffer, at pH 10.2, at 25°C
0.016
NAD+
mutant enzyme D249A, in 0.1 M CAPS buffer, at pH 10.2, at 25°C
0.4
NADH
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cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
0.91
NADPH
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cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
1.33
NADPH
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microsomal NADPH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
0.119
testosterone
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pH 7.4, 35°C, 3alpha-hydroxysteroid dehydrogenase activity
additional information
additional information
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values for various substrates and different isozymes
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additional information
additional information
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steady-state kinetics
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additional information
additional information
steady-state kinetics
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additional information
additional information
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kinetic mechanism, substrate binding mechanism of isozyme AKR1C2
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additional information
additional information
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kinetic mechanism, substrate binding mechanism of isozyme AKR1C9, wild-type and mutant enzymes
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additional information
additional information
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kinetic modeling of an enzymatic cycling reaction
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additional information
additional information
steady state kinetics, overview
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additional information
additional information
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steady state kinetics, overview
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additional information
additional information
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steady-state kinetics, ternary complex, sequential, kinetic mechanism of allopregnanolone synthesis with the cofactor binding before the steroid and leaving after it
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additional information
additional information
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equilibrium kinetics of wild-type and mutant enzymes
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additional information
additional information
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transient single and multiple turnover kinetics, stopped flow kinetics, isotope kinetics, ligand binding analysis, kinetics with deuterium-substituted cofactor and binary complex of enzyme and cofactor, overview
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additional information
additional information
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cofactor kinetics of wild-type and mutant enzymes, overview
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additional information
additional information
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kinetics in absence and presence of co-solvents, overview
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additional information
additional information
kinetics of wild-type and mutant enzymes in presence or absence of CAPS and methylamine, overview
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additional information
additional information
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steady-state, transient state kinetics, and kinetic isotope effects, kinetic analysis and mechanism, ordered bi-bi mechanism, detailed overview
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additional information
additional information
steady-state kinetics, and stopped-flow study, kinetics of enzyme mutants P185A, P185G, T188A, and T188S showing an increase in kcat, Ka drosterone and KiNAD and equal primary isotope effects of DV and D(V/K)
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