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7.3
2,3-diketo-L-gulonate
-
cofactor NADH, pH 6.3
0.45
beta-keto-L-gulonate
-
cofactor NADH, pH 6.3
19
D-gluconate
-
cofactor NAD+, pH 8.5
5.3 - 22.8
L-3-hydroxybutanoate
2.2 - 2.3
L-3-hydroxybutyrate
5.3
L-Idonate
-
cofactor NAD+, pH 8.5
additional information
additional information
-
6.4
acetoacetate
-
cofactor NADH, pH 6.3
8.3
acetoacetate
-
at pH 8.5, rabbit liver GDH
9.9
acetoacetate
-
at pH 8.5, recombinant rabbit GDH
5.3
L-3-hydroxybutanoate
-
-
14.9
L-3-hydroxybutanoate
-
cofactor NAD+, pH 8.2
22.8
L-3-hydroxybutanoate
-
cofactor NAD+
2.2
L-3-hydroxybutyrate
-
at pH 8.5, recombinant rabbit GDH
2.3
L-3-hydroxybutyrate
-
at pH 8.5, rabbit liver GDH
0.018
L-gulonate
with NAD+, wild-type enzyme, pH 7.0, 25°C
0.18
L-gulonate
-
at pH 8.5, recombinant rabbit GDH
0.21
L-gulonate
-
at pH 8.5, rabbit liver GDH
0.25
L-gulonate
-
cofactor NAD+
0.49
L-gulonate
-
mutant enzyme E157Q, NAD+-linked activity
0.85
L-gulonate
-
cofactor NAD+, pH 8.2
1.3
L-gulonate
with NAD+, mutant enzyme Q41N, pH 7.0, 25°C
1.6
L-gulonate
with NAD+, mutant enzyme E97Q, pH 7.0, 25°C
2.4
L-gulonate
with NADP+, wild-type enzyme, pH 7.0, 25°C
2.6
L-gulonate
with NADP+, mutant enzyme D36R/Q41N, pH 7.0, 25°C
3.3
L-gulonate
with NAD+, mutant enzyme D36R/Q41N, pH 7.0, 25°C
4.4
L-gulonate
-
mutant enzyme S124A, NAD+-linked activity
5.1
L-gulonate
-
cofactor NAD+, pH 8.5
14
L-gulonate
-
mutant enzyme D36R, NAD+-linked activity
14
L-gulonate
with NAD+, mutant enzyme D36R, pH 7.0, 25°C
14
L-gulonate
with NADP+, mutant enzyme Q41N, pH 7.0, 25°C
18
L-gulonate
with NADP+, mutant enzyme D36R, pH 7.0, 25°C
18.5
L-gulonate
-
mutant enzyme D36R, NADP+-linked activity
18.5
L-gulonate
-
mutant enzyme E157Q, NADP+-linked activity
55
L-gulonate
-
cofactor NAD+, pH 8.5
67
L-gulonate
-
cofactor NAD+, pH 7.5
1.2
L-threonate
-
at pH 8.5, recombinant rabbit GDH
2.9
L-threonate
-
at pH 8.5, rabbit liver GDH
0.01
NAD+
wild-type enzyme, pH 7.0, 25°C
0.01
NAD+
-
at pH 8.5, recombinant rabbit GDH
0.011
NAD+
-
at pH 8.5, rabbit liver GDH
0.028
NAD+
mutant enzyme Q41N, pH 7.0, 25°C
0.04
NAD+
-
cosubstrate L-gulonate, pH 8.5
0.054
NAD+
mutant enzyme E97Q, pH 7.0, 25°C
0.09
NAD+
-
cosubstrate L-gulonate, pH 8.2
0.13
NAD+
-
cosubstrate L-gulonate, pH 8.2
1.1
NAD+
-
cosubstrate L-gulonate, pH 7.5 and pH 8.5
1.3
NAD+
mutant enzyme D36R, pH 7.0, 25°C
2.6
NAD+
mutant enzyme D36R/Q41N, pH 7.0, 25°C
0.0005
NADH
-
at pH 8.5, rabbit liver GDH
0.0006
NADH
-
at pH 8.5, recombinant rabbit GDH
0.12
NADP+
mutant enzyme D36R/Q41N, pH 7.0, 25°C
0.2
NADP+
mutant enzyme D36R, pH 7.0, 25°C
0.67
NADP+
wild-type enzyme, pH 7.0, 25°C
0.67
NADP+
-
at pH 8.5, recombinant rabbit GDH
0.86
NADP+
mutant enzyme Q41N, pH 7.0, 25°C
0.89
NADP+
-
at pH 8.5, rabbit liver GDH
additional information
additional information
-
kinetic studies
-
additional information
additional information
-
kinetic studies
-
additional information
additional information
-
no activity with 5-40 mM sugars or hydroxyacids like 2-keto-L-guluonate, 3-deoxyglucosone, xylitol, L-threitol, ascorbic acid, malic acid, L-threonine, L-glycerate, L-tartarate and L-lactate, or 50 mM ethyl and methyl esters of 3-hydroxybutyrate
-
1.1.1.45 L-gulonate 3-dehydrogenase
results (
results (
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