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1.1.1.428: 4-methylthio 2-oxobutanoate reductase (NADH)

This is an abbreviated version!
For detailed information about 4-methylthio 2-oxobutanoate reductase (NADH), go to the full flat file.

Reaction

(2R)-2-hydroxy-4-(methylsulfanyl)butanoate
+
NAD+
=
4-(methylsulfanyl)-2-oxobutanoate
+
NADH
+
H+

Synonyms

4-methylthio 2-oxobutyrate reductase, 4-methylthio 2-oxobutyric acid reductase, C-terminal binding protein 1, C-terminal-binding protein 1, C-terminal-binding protein 2, CtBP, CTBP1, CtBP2, MTOB reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.428 4-methylthio 2-oxobutanoate reductase (NADH)

KCat KM Value

KCat KM Value on EC 1.1.1.428 - 4-methylthio 2-oxobutanoate reductase (NADH)

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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.677
2-oxocaproate
-
at pH 7.1 and 30°C
0.012
2-oxogluconate
-
at pH 7.1 and 30°C
0.049
2-oxoglutarate
-
at pH 7.1 and 30°C
0.228
2-oxoisocaproate
-
at pH 7.1 and 30°C
0.103
2-oxovalerate
-
at pH 7.1 and 30°C
0.0084
3-phosphohydroxypyruvate
-
at pH 7.1 and 30°C
5.28
4-(methylsulfanyl)-2-oxobutanoate
-
at pH 7.1 and 30°C
0.28
glyoxylate
-
at pH 7.1 and 30°C
0.019
oxaloacetate
-
at pH 7.1 and 30°C
0.273
phenylpyruvate
-
at pH 7.1 and 30°C
0.065
pyruvate
-
at pH 7.1 and 30°C